JCB logo
Carestream Gel Logic 212PRO
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 21 April 2003. doi:10.1083/jcb.200210130
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 514K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vizeacoumar, F. J.
Right arrow Articles by Rachubinski, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vizeacoumar, F. J.
Right arrow Articles by Rachubinski, R. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

© The Rockefeller University Press, 0021-9525/2003/4/321 $5.00
The Journal of Cell Biology, Volume 161, Number 2, 321-332

Article

YHR150w and YDR479c encode peroxisomal integral membrane proteins involved in the regulation of peroxisome number, size, and distribution in Saccharomyces cerevisiae



Franco J. Vizeacoumar1, Juan C. Torres-Guzman1,2, Yuen Yi C. Tam1, John D. Aitchison1,3 and Richard A. Rachubinski1

1 Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
2 Instituto de Investigaciones en Biologiá Experimental, University of Guanajuato, Guanajuato, Mexico
3 The Institute for Systems Biology, Seattle, WA 98103

Address correspondence to Richard A. Rachubinski, Department of Cell Biology, University of Alberta, Medical Sciences Building 5-14, Edmonton, Alberta T6G 2H7, Canada. Tel: (780) 492-9868. Fax: (780) 492-9278. E-mail: rick.rachubinski{at}ualberta.ca

The peroxin Pex24p of the yeast Yarrowia lipolytica exhibits high sequence similarity to two hypothetical proteins, Yhr150p and Ydr479p, encoded by the Saccharomyces cerevisiae genome. Like YlPex24p, both Yhr150p and Ydr479p have been shown to be integral to the peroxisomal membrane, but unlike YlPex24p, their levels of synthesis are not increased upon a shift of cells from glucose- to oleic acid–containing medium. Peroxisomes of cells deleted for either or both of the YHR150w and YDR479c genes are increased in number, exhibit extensive clustering, are smaller in area than peroxisomes of wild-type cells, and often exhibit membrane thickening between adjacent peroxisomes in a cluster. Peroxisomes isolated from cells deleted for both genes have a decreased buoyant density compared with peroxisomes isolated from wild-type cells and still exhibit clustering and peroxisomal membrane thickening. Overexpression of the genes PEX25 or VPS1, but not the gene PEX11, restored the wild-type phenotype to cells deleted for one or both of the YHR150w and YDR479c genes. Together, our data suggest a role for Yhr150p and Ydr479p, together with Pex25p and Vps1p, in regulating peroxisome number, size, and distribution in S. cerevisiae. Because of their role in peroxisome dynamics, YHR150w and YDR479c have been designated as PEX28 and PEX29, respectively, and their encoded peroxins as Pex28p and Pex29p.

Key Words: biogenesis; peroxin; protein similarity; open reading frame; membrane fission

The online version of this article includes supplemental material.

* Abbreviations used in this paper: DsRed, Discosoma sp. red fluorescent protein; PBD, peroxisome biogenesis disorder; PNS, postnuclear supernatant; PTS1, peroxisome-targeting signal 1; SM, synthetic minimal.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents