Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin

doi:10.1083/jcb.200303012

Published online 19 May 2003. doi:10.1083/jcb.200303012

This Article

	Full Text
	Full Text (PDF, 343K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Piacentini, L.
	Articles by Pimpinelli, S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Piacentini, L.
	Articles by Pimpinelli, S.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2003/5/707 $5.00
The Journal of Cell Biology, Volume 161, Number 4, 707-714

Article

Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin

Lucia Piacentini¹, Laura Fanti¹, Maria Berloco², Barbara Perrini¹ and Sergio Pimpinelli¹

¹ Istituto Pasteur, Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Università di Roma "La Sapienza," 00185 Roma, Italy
² Dipartimento di Anatomia Patologica e di Genetica, Università di Bari, 70126 Bari, Italy

Address correspondence to Sergio Pimpinelli, Dipartimento di Genetica e Biologia Molecolare, Università di Roma "La Sapienza," Piazzale Aldo Moro, 5, 00185 Roma, Italy. Tel.: 39-6-49912876. Fax: 39-6-4456866. E-mail: sergio.pimpinelli{at}uniroma1.it

Heterochromatin protein 1 (HP1) is a conserved nonhistone chromosomalprotein, which is involved in heterochromatin formation andgene silencing in many organisms. In addition, it has been shownthat HP1 is also involved in telomere capping in Drosophila.Here, we show a novel striking feature of this protein demonstratingits involvement in the activation of several euchromatic genesin Drosophila. By immunostaining experiments using an HP1 antibody,we found that HP1 is associated with developmental and heatshock–induced puffs on polytene chromosomes. Because thepuffs are the cytological phenotype of intense gene activity,we did a detailed analysis of the heat shock–induced expressionof the HSP70 encoding gene in larvae with different doses ofHP1 and found that HP1 is positively involved in Hsp70 geneactivity. These data significantly broaden the current viewsof the roles of HP1 in vivo by demonstrating that this proteinhas multifunctional roles.

Key Words: HP1; heterochromatin; HSP70; Drosophila; euchromatin

^* Abbreviations used in this paper: HP1, heterochromatin protein1; HSE, heat shock factor binding sites; HSF, heat shock factor;X-ChIP, formaldehyde cross-linked chromatin immunoprecipitation.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Fischer, T., Cui, B., Dhakshnamoorthy, J., Zhou, M., Rubin, C., Zofall, M., Veenstra, T. D., Grewal, S. I. S. (2009). Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast. Proc. Natl. Acad. Sci. USA 106: 8998-9003 [Abstract] [Full Text]
Muhonen, P., Holthofer, H. (2009). Epigenetic and microRNA-mediated regulation in diabetes. Nephrol Dial Transplant 24: 1088-1096 [Full Text]
Sadaie, M., Kawaguchi, R., Ohtani, Y., Arisaka, F., Tanaka, K., Shirahige, K., Nakayama, J.-i. (2008). Balance between Distinct HP1 Family Proteins Controls Heterochromatin Assembly in Fission Yeast. Mol. Cell. Biol. 28: 6973-6988 [Abstract] [Full Text]
Font-Burgada, J., Rossell, D., Auer, H., Azorin, F. (2008). Drosophila HP1c isoform interacts with the zinc-finger proteins WOC and Relative-of-WOC to regulate gene expression. Genes Dev. 22: 3007-3023 [Abstract] [Full Text]
Suzuki, K., Juelich, T., Lim, H., Ishida, T., Watanebe, T., Cooper, D. A., Rao, S., Kelleher, A. D. (2008). Closed Chromatin Architecture Is Induced by an RNA Duplex Targeting the HIV-1 Promoter Region. J. Biol. Chem. 283: 23353-23363 [Abstract] [Full Text]
Brower-Toland, B., Findley, S. D., Jiang, L., Liu, L., Yin, H., Dus, M., Zhou, P., Elgin, S. C.R., Lin, H. (2007). Drosophila PIWI associates with chromatin and interacts directly with HP1a. Genes Dev. 21: 2300-2311 [Abstract] [Full Text]
Gazzar, M. E., Yoza, B. K., Hu, J. Y.-Q., Cousart, S. L., McCall, C. E. (2007). Epigenetic Silencing of Tumor Necrosis Factor {alpha} during Endotoxin Tolerance. J. Biol. Chem. 282: 26857-26864 [Abstract] [Full Text]
Bongiorni, S., Pasqualini, B., Taranta, M., Singh, P. B., Prantera, G. (2007). Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3-HP1-Me(3)K20H4 pathway. J. Cell Sci. 120: 1072-1080 [Abstract] [Full Text]
Vogel, M. J., Guelen, L., de Wit, E., Hupkes, D. P., Loden, M., Talhout, W., Feenstra, M., Abbas, B., Classen, A.-K., van Steensel, B. (2006). Human heterochromatin proteins form large domains containing KRAB-ZNF genes. Genome Res 16: 1493-1504 [Abstract] [Full Text]
Teyssier, C., Ou, C.-Y., Khetchoumian, K., Losson, R., Stallcup, M. R. (2006). Transcriptional Intermediary Factor 1{alpha} Mediates Physical Interaction and Functional Synergy between the Coactivator-Associated Arginine Methyltransferase 1 and Glucocorticoid Receptor-Interacting Protein 1 Nuclear Receptor Coactivators. Mol. Endocrinol. 20: 1276-1286 [Abstract] [Full Text]
Zhang, W., Deng, H., Bao, X., Lerach, S., Girton, J., Johansen, J., Johansen, K. M. (2006). The JIL-1 histone H3S10 kinase regulates dimethyl H3K9 modifications and heterochromatic spreading in Drosophila. Development 133: 229-235 [Abstract] [Full Text]
Zemach, A., Li, Y., Ben-Meir, H., Oliva, M., Mosquna, A., Kiss, V., Avivi, Y., Ohad, N., Grafi, G. (2006). Different Domains Control the Localization and Mobility of LIKE HETEROCHROMATIN PROTEIN1 in Arabidopsis Nuclei. Plant Cell 18: 133-145 [Abstract] [Full Text]
Spierer, A., Seum, C., Delattre, M., Spierer, P. (2005). Loss of the modifiers of variegation Su(var)3-7 or HP1 impacts male X polytene chromosome morphology and dosage compensation. J. Cell Sci. 118: 5047-5057 [Abstract] [Full Text]
De Lucia, F., Ni, J.-Q., Vaillant, C., Sun, F.-L. (2005). HP1 modulates the transcription of cell-cycle regulators in Drosophila melanogaster. Nucleic Acids Res 33: 2852-2858 [Abstract] [Full Text]
Marchion, D. C., Bicaku, E., Daud, A. I., Sullivan, D. M., Munster, P. N. (2005). Valproic Acid Alters Chromatin Structure by Regulation of Chromatin Modulation Proteins. Cancer Res. 65: 3815-3822 [Abstract] [Full Text]
Lee, D. Y., Teyssier, C., Strahl, B. D., Stallcup, M. R. (2005). Role of Protein Methylation in Regulation of Transcription. Endocr. Rev. 26: 147-170 [Abstract] [Full Text]
Schulze, S. R., Sinclair, D. A. R., Fitzpatrick, K. A., Honda, B. M. (2005). A Genetic and Molecular Characterization of Two Proximal Heterochromatic Genes on Chromosome 3 of Drosophila melanogaster. Genetics 169: 2165-2177 [Abstract] [Full Text]
Beckstead, R. B., Ner, S. S., Hales, K. G., Grigliatti, T. A., Baker, B. S., Bellen, H. J. (2005). Bonus, a Drosophila TIF1 Homolog, Is a Chromatin-Associated Protein That Acts as a Modifier of Position-Effect Variegation. Genetics 169: 783-794 [Abstract] [Full Text]
Oh, H. J., Li, Y., Lau, Y.-F. C. (2005). Sry Associates with the Heterochromatin Protein 1 Complex by Interacting with a KRAB Domain Protein. Biol. Reprod. 72: 407-415 [Abstract] [Full Text]
Bushey, D., Locke, J. (2004). Mutations in Su(var)205 and Su(var)3-7 Suppress P-Element-Dependent Silencing in Drosophila melanogaster. Genetics 168: 1395-1411 [Abstract] [Full Text]
Makatsori, D., Kourmouli, N., Polioudaki, H., Shultz, L. D., Mclean, K., Theodoropoulos, P. A., Singh, P. B., Georgatos, S. D. (2004). The Inner Nuclear Membrane Protein Lamin B Receptor Forms Distinct Microdomains and Links Epigenetically Marked Chromatin to the Nuclear Envelope. J. Biol. Chem. 279: 25567-25573 [Abstract] [Full Text]
Schmiedeberg, L., Weisshart, K., Diekmann, S., Meyer zu Hoerste, G., Hemmerich, P. (2004). High- and Low-mobility Populations of HP1 in Heterochromatin of Mammalian Cells. Mol. Biol. Cell 15: 2819-2833 [Abstract] [Full Text]
Lara-Pezzi, E., Pezzi, N., Prieto, I., Barthelemy, I., Carreiro, C., Martinez, A., Maldonado-Rodriguez, A., Lopez-Cabrera, M., Barbero, J. L. (2004). Evidence of a Transcriptional Co-activator Function of Cohesin STAG/SA/Scc3. J. Biol. Chem. 279: 6553-6559 [Abstract] [Full Text]
Sharma, G. G., Hwang, K.-k., Pandita, R. K., Gupta, A., Dhar, S., Parenteau, J., Agarwal, M., Worman, H. J., Wellinger, R. J., Pandita, T. K. (2003). Human Heterochromatin Protein 1 Isoforms HP1Hs{alpha} and HP1Hs{beta} Interfere with hTERT-Telomere Interactions and Correlate with Changes in Cell Growth and Response to Ionizing Radiation. Mol. Cell. Biol. 23: 8363-8376 [Abstract] [Full Text]
Greil, F., van der Kraan, I., Delrow, J., Smothers, J. F., de Wit, E., Bussemaker, H. J., van Driel, R., Henikoff, S., van Steensel, B. (2003). Distinct HP1 and Su(var)3-9 complexes bind to sets of developmentally coexpressed genes depending on chromosomal location. Genes Dev. 17: 2825-2838 [Abstract] [Full Text]
Sharp, J. A., Krawitz, D. C., Gardner, K. A., Fox, C. A., Kaufman, P. D. (2003). The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin. Genes Dev. 17: 2356-2361 [Abstract] [Full Text]
Kellum, R. (2003). Is HP1 an RNA detector that functions both in repression and activation?. JCB 161: 671-672 [Abstract] [Full Text]