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Published online 22 September 2003. doi:10.1083/jcb.200305055
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© The Rockefeller University Press, 0021-9525/2003/9/1255 $5.00
The Journal of Cell Biology, Volume 162, Number 7, 1255-1266

Article

Peroxisome division in the yeast Yarrowia lipolytica is regulated by a signal from inside the peroxisome



Tong Guo1, Yuriy Y. Kit1, Jean-Marc Nicaud2, Marie-Therese Le Dall2, S. Kelly Sears3, Hojatollah Vali3, Honey Chan4, Richard A. Rachubinski4 and Vladimir I. Titorenko1

1 Department of Biology, Concordia University, Montreal, Quebec H4B 1R6, Canada
2 Laboratoire de Génétique des Microorganismes, 78850 Thiverval-Grignon, France
3 Facility for Electron Microscopy Research, McGill University, Montreal, Quebec H3A 2B2, Canada
4 Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

Address correspondence to Vladimir I. Titorenko, Department of Biology, Concordia University, 7141 Sherbrooke Street, West, Montreal, Quebec H4B 1R6, Canada. Tel.: (514) 848-2424-3424. Fax: (514) 848-2424-2881. email: VTITOR{at}vax2.concordia.ca

We describe an unusual mechanism for organelle division. In the yeast Yarrowia lipolytica, only mature peroxisomes contain the complete set of matrix proteins. These mature peroxisomes assemble from several immature peroxisomal vesicles in a multistep pathway. The stepwise import of distinct subsets of matrix proteins into different immature intermediates along the pathway causes the redistribution of a peroxisomal protein, acyl-CoA oxidase (Aox), from the matrix to the membrane. A significant redistribution of Aox occurs only in mature peroxisomes. Inside mature peroxisomes, the membrane-bound pool of Aox interacts with Pex16p, a membrane-associated protein that negatively regulates the division of early intermediates in the pathway. This interaction inhibits the negative action of Pex16p, thereby allowing mature peroxisomes to divide.

Key Words: organelle division; membrane fission; peroxisome assembly; peroxin; liposomes

The online version of this article includes supplemental material.

Abbreviations used in this paper: Aox, acyl-CoA oxidase; ICL, isocitrate lyase; MLS, malate synthase; PIC, protease inhibitor cocktail; PMP, peroxisomal membrane protein; THI, thiolase.


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