A J-protein is an essential subunit of the presequence translocase-associated protein import motor of mitochondria

doi:10.1083/jcb.200308004

Published 24 November 2003. doi:10.1083/jcb.200308004

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© The Rockefeller University Press, 0021-9525/2003/11/707 $8.00
The Journal of Cell Biology, Volume 163, Number 4, 707-713

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A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria

Kaye N. Truscott¹, Wolfgang Voos¹, Ann E. Frazier¹^,2, Maria Lind¹, Yanfeng Li¹^,2, Andreas Geissler¹, Jan Dudek¹^,2, Hanne Müller¹, Albert Sickmann³^,4, Helmut E. Meyer³, Chris Meisinger¹, Bernard Guiard⁵, Peter Rehling¹ and Nikolaus Pfanner¹

¹ Institut für Biochemie und Molekularbiologie, Universität Freiburg, D-79104 Freiburg, Germany
² Fakultät für Biologie, Universität Freiburg, D-79104 Freiburg, Germany
³ Medizinisches Proteom-Center, Ruhr-Universität Bochum, D-44780 Bochum, Germany
⁴ Rudolf-Virchow-Center for Experimental Biomedicine, Universität Würzburg, D-97078 Würzburg, Germany
⁵ Laboratoire propre du CNRS Université Pierre et Marie Curie, 91190 Gif-sur-Yvette, France

Address correspondence to Nikolaus Pfanner, Institut für Biochemie und Molekularbiologie, Universität Freiburg, Hermann-Herder-Strasse 7, D-79104 Freiburg, Germany. Tel.: 49-761-203-5224. Fax: 49-761-203-5261. email: Nikolaus.Pfanner{at}biochemie.uni-freiburg.de

Transport of preproteins into the mitochondrial matrix is mediatedby the presequence translocase–associated motor (PAM).Three essential subunits of the motor are known: mitochondrialHsp70 (mtHsp70); the peripheral membrane protein Tim44; andthe nucleotide exchange factor Mge1. We have identified thefourth essential subunit of the PAM, an essential inner membraneprotein of 18 kD with a J-domain that stimulates the ATPaseactivity of mtHsp70. The novel J-protein (encoded by PAM18/YLR008c/TIM14)is required for the interaction of mtHsp70 with Tim44 and proteintranslocation into the matrix. We conclude that the reactioncycle of the PAM of mitochondria involves an essential J-protein.

Key Words: Hsp70; J-protein; mitochondria; protein translocation; Saccharomyces cerevisiae

K.N. Truscott and W. Voos contributed equally to this work.

Abbreviations used in this paper: ${Delta}$ ${psi}$ , membrane potential; mtHsp70,mitochondrial Hsp70; PAM, presequence translocase–associatedmotor.

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