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Published online 1 December 2003. doi:10.1083/jcb.200306079
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© The Rockefeller University Press, 0021-9525/2003/12/1089 $8.00
The Journal of Cell Biology, Volume 163, Number 5, 1089-1098

Article

Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia



Melissa M. Rolls, Roger Albertson, Hsin-Pei Shih, Cheng-Yu Lee and Chris Q. Doe

Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403

Address correspondence to Chris Q. Doe, Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, 1254 University of Oregon, Eugene, OR 97403. Tel.: (541) 346-4877. Fax: (541) 346-4736. email: cdoe{at}uoneuro.uoregon.edu

Cell polarity is essential for generating cell diversity and for the proper function of most differentiated cell types. In many organisms, cell polarity is regulated by the atypical protein kinase C (aPKC), Bazooka (Baz/Par3), and Par6 proteins. Here, we show that Drosophila aPKC zygotic null mutants survive to mid-larval stages, where they exhibit defects in neuroblast and epithelial cell polarity. Mutant neuroblasts lack apical localization of Par6 and Lgl, and fail to exclude Miranda from the apical cortex; yet, they show normal apical crescents of Baz/Par3, Pins, Inscuteable, and Discs large and normal spindle orientation. Mutant imaginal disc epithelia have defects in apical/basal cell polarity and tissue morphology. In addition, we show that aPKC mutants show reduced cell proliferation in both neuroblasts and epithelia, the opposite of the lethal giant larvae (lgl) tumor suppressor phenotype, and that reduced aPKC levels strongly suppress most lgl cell polarity and overproliferation phenotypes.

Key Words: Lgl; asymmetric cell division; Miranda; apical/basal polarity; Par complex

M. Rolls and R. Albertson contributed equally to this paper.

Abbreviations used in this paper: aPKC, atypical protein kinase C; Dlg, Discs large; Ecad, E-cadherin; GMC, ganglion mother cell; Insc, Inscuteable; Lgl, Lethal giant larvae; Scrib, Scribble.


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