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© The Rockefeller University Press,
0021-9525/2003/12/1145 $8.00
The Journal of Cell Biology, Volume 163, Number 5, 1145-1155
Article |
Peritoneal macrophages express both P-selectin and PSGL-1
Address correspondence to Barbara C. Furie, Center for Hemostasis, Thrombosis and Vascular Biology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave., Boston, MA 02215. Tel.: (617) 667-0620. Fax: (617) 975-5505. email: bfurie1{at}bidmc.harvard.edu
Macrophages, phagocytic cells involved in an early phase of host defense, are known to express the P-selectin ligand, PSGL-1. Heretofore, P-selectin has only been found on platelets and endothelial cells. Here, we demonstrate that peritoneal macrophages isolated by peritoneal lavage of unchallenged mice express P-selectin on the plasma membrane. The peritoneal macrophages synthesize P-selectin, as indicated by metabolic labeling experiments. P-Selectin is constitutively expressed on the extracellular surface of macrophages but is only partially colocalized with PSGL-1. P-Selectin is rapidly translocated from the macrophage plasma membrane to intracellular vesicles and to lysosomes. Peritoneal macrophages assemble into cell strings under flow conditions based upon macrophage–macrophage interactions mediated by P-selectin and PSGL-1. This is the first description of a leukocyte shown to express both P-selectin and PSGL-1.
Key Words: secondary interactions; endocytosis; trafficking; lymphatics
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