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Published 8 December 2003. doi:10.1083/jcb.200308071
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© The Rockefeller University Press, 0021-9525/2003/12/973 $8.00
The Journal of Cell Biology, Volume 163, Number 5, 973-985

Article

Yeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage



Chao-Wen Wang1, Per E. Stromhaug1, Emily J. Kauffman2, Lois S. Weisman2 and Daniel J. Klionsky1

1 Life Sciences Institute and the Department of Molecular, Cellular and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109
2 Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242

Address correspondence to Daniel J. Klionsky, University of Michigan, Life Sciences Institute, Ann Arbor, MI 48109-2216. Tel.: (734) 615-6556. Fax: (734) 647-0884. email: klionsky{at}umich.edu

The function of the yeast lysosome/vacuole is critically linked with the morphology of the organelle. Accordingly, highly regulated processes control vacuolar fission and fusion events. Analysis of homotypic vacuole fusion demonstrated that vacuoles from strains defective in the CCZ1 and MON1 genes could not fuse. Morphological evidence suggested that these mutant vacuoles could not proceed to the tethering/docking stage. Ccz1 and Mon1 form a stable protein complex that binds the vacuole membrane. In the absence of the Ccz1–Mon1 complex, the integrity of vacuole SNARE pairing and the unpaired SNARE class C Vps/HOPS complex interaction were both impaired. The Ccz1–Mon1 complex colocalized with other fusion components on the vacuole as part of the cis-SNARE complex, and the association of the Ccz1–Mon1 complex with the vacuole appeared to be regulated by the class C Vps/HOPS complex proteins. Accordingly, we propose that the Ccz1–Mon1 complex is critical for the Ypt7-dependent tethering/docking stage leading to the formation of a trans-SNARE complex and subsequent vacuole fusion.

Key Words: class C Vps; HOPS; membrane fusion; Rab; SNAREs

Abbreviations used in this paper: AD, activation domain; ARS, ATP regeneration system; BD, binding domain; C-Vps, class C Vps; Cvt, cytoplasm-to-vacuole targeting; PA, protein A; SMD, synthetic minimal medium with dextrose.


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