Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae

doi:10.1083/jcb.200304030

Published 22 December 2003. doi:10.1083/jcb.200304030

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© The Rockefeller University Press, 0021-9525/2003/12/1255 $8.00
The Journal of Cell Biology, Volume 163, Number 6, 1255-1266

Article

Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae

Paula Estrada¹^,2, Jiwon Kim¹^,2, Jeff Coleman², Lee Walker¹^,2, Brian Dunn², Peter Takizawa², Peter Novick² and Susan Ferro-Novick¹^,2

¹ Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06519
² Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06519

Address correspondence to Susan Ferro-Novick, Department of Cell Biology, Yale University School of Medicine, Boyer Center for Molecular Medicine, Howard Hughes Medical Institute, 295 Congress Ave., Room 254B, New Haven, CT 06519-1418. Tel.: (203) 737-5207. Fax: (203) 737-5746. email: susan.ferronovick{at}yale.edu

Myo4p is a nonessential type V myosin required for the bud tiplocalization of ASH1 and IST2 mRNA. These mRNAs associate withMyo4p via the She2p and She3p proteins. She3p is an adaptorprotein that links Myo4p to its cargo. She2p binds to ASH1 andIST2 mRNA, while She3p binds to both She2p and Myo4p. Here weshow that Myo4p and She3p, but not She2p, are required for theinheritance of cortical ER in the budding yeast Saccharomycescerevisiae. Consistent with this observation, we find that corticalER inheritance is independent of mRNA transport. Cortical ERis a dynamic network that forms cytoplasmic tubular connectionsto the nuclear envelope. ER tubules failed to grow when actinpolymerization was blocked with the drug latrunculin A (Lat-A).Additionally, a reduction in the number of cytoplasmic ER tubuleswas observed in Lat-A–treated and myo4 ${Delta}$ cells. Our resultssuggest that Myo4p and She3p facilitate the growth and orientationof ER tubules.

Key Words: cortical ER inheritance; Myo4p; She proteins; myosin; yeast

P. Estrada and J. Kim contributed equally to this paper.

The online version of this article includes supplemental material.

Abbreviations used in this paper: Lat-A, latrunculin A; SC,synthetic complete.

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