JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 29 December 2003. doi:10.1083/jcb.200307098
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 1, 133-144
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1271K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Benaud, C.
Right arrow Articles by Baudier, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Benaud, C.
Right arrow Articles by Baudier, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 AHNAK interaction with the annexin 2/S100A10 complex regulates cell membrane cytoarchitecture

Christelle Benaud1, Benoît J. Gentil1, Nicole Assard1, Magalie Court2, Jerome Garin2, Christian Delphin1 and Jacques Baudier1

1 Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
2 Laboratoire de Chimie des Proteines, INSERM ERM-0201, DRDC-CP, CEA-Grenoble, 38054 Grenoble Cedex 9, France

Address correspondence to Jacques Baudier, INSERM EMI-0104, DRDC-TS, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France. Tel.: (33) 4-38-78-43 28. Fax: (33) 4-38-78-50-58. email: jbaudier{at}cea.fr

Remodelling of the plasma membrane cytoarchitecture is crucial for the regulation of epithelial cell adhesion and permeability. In Madin-Darby canine kidney cells, the protein AHNAK relocates from the cytosol to the cytosolic surface of the plasma membrane during the formation of cell–cell contacts and the development of epithelial polarity. This targeting is reversible and regulated by Ca2+-dependent cell–cell adhesion. At the plasma membrane, AHNAK associates as a multimeric complex with actin and the annexin 2/S100A10 complex. The S100A10 subunit serves to mediate the interaction between annexin 2 and the COOH-terminal regulatory domain of AHNAK. Down-regulation of both annexin 2 and S100A10 using an annexin 2–specific small interfering RNA inhibits the association of AHNAK with plasma membrane. In Madin-Darby canine kidney cells, down-regulation of AHNAK using AHNAK-specific small interfering RNA prevents cortical actin cytoskeleton reorganization required to support cell height. We propose that the interaction of AHNAK with the annexin 2/S100A10 regulates cortical actin cytoskeleton organization and cell membrane cytoarchitecture.

Key Words: actin; calcium; cytoskeleton; cell adhesion; S100B

The online version of this article includes supplemental material.

Abbreviation used in this paper: siRNA, small interfering RNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents