JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

Published online 12 January 2004. doi:10.1083/jcb.200310027
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 2, 169-174
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 493K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iioka, H.
Right arrow Articles by Kinoshita, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iioka, H.
Right arrow Articles by Kinoshita, N.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Report

Essential role of MARCKS in cortical actin dynamics during gastrulation movements



Hidekazu Iioka, Naoto Ueno, and Noriyuki Kinoshita

Department of Developmental Biology, National Institute for Basic Biology, and Department of Molecular Biomechanics, The Graduate University for Advanced Studies, Aichi 444-8585, Japan

Address correspondence to Noriyuki Kinoshita, Dept. of Developmental Biology, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji Okazaki, Aichi 444-8585, Japan. Tel.: 81-564-55-7573. Fax: 81-564-55-7571. email: nkinoshi{at}nibb.ac.jp

Myristoylated alanine-rich C kinase substrate (MARCKS) is an actin-binding, membrane-associated protein expressed during Xenopus embryogenesis. We analyzed its function in cytoskeletal regulation during gastrulation. Here, we show that blockade of its function impaired morphogenetic movements, including convergent extension. MARCKS was required for control of cell morphology, motility, adhesion, protrusive activity, and cortical actin formation in embryonic cells. We also demonstrate that the noncanonical Wnt pathway promotes the formation of lamellipodia- and filopodia-like protrusions and that MARCKS is necessary for this activity. These findings show that MARCKS regulates the cortical actin formation that is requisite for dynamic morphogenetic movements.

Key Words: cell adhesion; Xenopus; cell movement; convergent extension; Wnt pathway


The online version of this paper includes supplemental material.

Abbreviations used in this paper: DMZ, dorsal marginal zone; FN, fibronectin; MARCKS, myristoylated alanine-rich C kinase substrate, mb-Venus, membrane-binding Venus; Mo, Morpholino oligonucleotide; RFP, red fluorescent protein; RMA, RFP-moesin actin; Xdsh, Xenopus Dishevelled; XMLP, MARCKS-like protein.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents