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Published online 9 February 2004. doi:10.1083/jcb.200307176
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 4, 581-591
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Article

In Candida albicans, the Nim1 kinases Gin4 and Hsl1 negatively regulate pseudohypha formation and Gin4 also controls septin organization



Raymond Wightman, Steven Bates, Pat Amornrrattanapan, and Peter Sudbery

Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, S10 2TN UK

Address correspondence to Peter Sudbery, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, S10 2TN UK. Tel.: 44-114-222-6186. Fax: 44-114-272-8697. email: psudbery{at}shef.ac.uk

In the development of hyphal germ tubes of Candida albicans, a band of septin forms at the base of the germ tube (basal septin band). Later, a septin ring forms, which organizes the first septum within the germ tube (septin ring). We have investigated the role of the Nim1 kinases, Gin4 and Hsl1, in the formation of these septin structures. We show that during germ tube formation, Gin4 is required for the organization of the septin ring but not the basal septin band. Hsl1 is not required for the formation of either septin rings or basal bands. Unexpectedly, we found that both gin4{Delta} and hsl1{Delta} mutants form pseudohyphae constitutively, in a fashion that in the case of gin4{Delta}, is partly independent of Swe1. Gin4-depleted pseudohyphae are unable to form hyphae when challenged with serum, but this can be overcome by ectopic expression of Gin4 from the MET3 promoter. Thus, Gin4 may regulate the developmental switch from pseudohyphae to hyphae.

Key Words: basal septin band; germ tube; hypha; septin ring; SWE1

R. Wightman and S. Bates contributed equally to this work.

The online version of this article contains supplemental material.

Steven Bates's present address is University of Aberdeen, Department of Molecular and Cell Biology, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD UK.

Abbreviations used in this paper: {alpha}Cdc11, antisera to S. cerevisiae Cdc11; IAA, isoamyl alcohol; YEPD, yeast extract peptone dextrose.


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