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Published online 23 February 2004. doi:10.1083/jcb.200310138
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 5, 647-652
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 Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body–specific localization signal

Beáta E. Jády1,2, Edouard Bertrand2, and Tamás Kiss1,3

1 Laboratoire de Biologie Moléculaire Eucaryote du CNRS, 31062 Toulouse, France
2 Institut de Génétique Moléculaire, 34000 Montpellier, France
3 Biological Research Center, Hungarian Academy of Sciences, 6726 Szeged, Hungary

Address correspondence to Tamás Kiss, Laboratoire de Biologie Moléculaire Eucaryote du CNRS, UMR5099, IFR109, Université Paul Sabatier, 118 Route de Narbonne, 31062 Toulouse, France. Tel.: (33) 5-61-33-59-91. Fax: (33) 5-61-33-58-86. email: tamas{at}ibcg.biotoul.fr

Telomerase is a ribonucleoprotein reverse transcriptase that uses its RNA component as a template for synthesis of telomeric DNA repeats at the ends of linear eukaryotic chromosomes. Here, fluorescence in situ hybridization demonstrates that in HeLa cancer cells, human telomerase RNA (hTR) accumulates in the nucleoplasmic Cajal bodies (CBs). Localization of transiently expressed hTR to CBs is supported by a short sequence motif (411-UGAG-414) that is located in the 3'-terminal box H/ACA RNA-like domain of hTR and that is structurally and functionally indistinguishable from the CB-specific localization signal of box H/ACA small CB-specific RNAs. In synchronized HeLa cells, hTR shows the most efficient accumulation in CBs during S phase, when telomeres are most likely synthesized. CBs may function in post-transcriptional maturation (e.g., cap hypermethylation of hTR), but they may also play a role in the assembly and/or function of telomerase holoenzyme.

Key Words: telomerase RNA; Cajal bodies; RNA localization; scaRNA; CAB box

Abbreviations used in this paper: CAB box, Cajal body box; CB, Cajal body; hTR, human telomerase RNA; pol II, RNA polymerase II; scaRNA, small Cajal body–specific RNA; SMN, survival of motor neurons; snoRNA, small nucleolar RNA; TMG, 2,2,7-trimethyl guanosine.


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