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Published 15 March 2004. doi:10.1083/jcb.200312133
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 6, 811-817
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Primary cilia of human endothelial cells disassemble under laminar shear stress



Carlo Iomini1, Karla Tejada1, Wenjun Mo3, Heikki Vaananen2, and Gianni Piperno1

1 Center for Anatomy and Functional Morphology, Mount Sinai School of Medicine, New York, NY 10029
2 Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029
3 Skirball Institute of Biomolecular Medicine and Department of Pharmacology, New York University School of Medicine, New York, NY 10016

Address correspondence to Gianni Piperno, Center for Anatomy and Functional Morphology, Mount Sinai School of Medicine, 1 Gustave L. Levy Pl., Box 1007, New York, NY 10029. Tel.: (212) 241-0773. Fax: (212) 860-1174. email: gianni.piperno{at}mssm.edu

We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-{alpha}-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriole at interphase and mitosis. To study the response of primary cilia and centrosomes to mechanical stimuli, we exposed cultured HUVEC to laminar shear stress (LSS). Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells.

Key Words: Chlamydomonas; IFT; HUVEC; CMG-1; PKD-1

W. Mo's present address is Analytical Biochemistry, Drug Discovery, Biogen Idec, Inc., 12 Cambridge Center, Cambridge, MA 02142.

Abbreviations used in this paper: CMG-1, capillary morphogenesis gene-1 product; HUVEC, human umbilical vein endothelial cells; IFT, intraflagellar transport; LSS, laminar shear stress.


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