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Published online 8 March 2004. doi:10.1083/jcb.200310077
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 6, 819-829
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Article

The cohesion protein ORD is required for homologue bias during meiotic recombination



Hayley A. Webber1, Louisa Howard2, and Sharon E. Bickel1

1 Department of Biological Sciences, Dartmouth College, Hanover, NH 03755
2 Ripple Electron Microscope Facility, Dartmouth College, Hanover, NH 03755

Address correspondence to S.E. Bickel, Dept. of Biological Sciences, Dartmouth College, 6044 Gilman, Hanover, NH 03755. Tel.: (603) 646-0245. Fax: (603) 646-1347. email: sharon.e.bickel{at}dartmouth.edu

During meiosis, sister chromatid cohesion is required for normal levels of homologous recombination, although how cohesion regulates exchange is not understood. Null mutations in orientation disruptor (ord) ablate arm and centromeric cohesion during Drosophila meiosis and severely reduce homologous crossovers in mutant oocytes. We show that ORD protein localizes along oocyte chromosomes during the stages in which recombination occurs. Although synaptonemal complex (SC) components initially associate with synapsed homologues in ord mutants, their localization is severely disrupted during pachytene progression, and normal tripartite SC is not visible by electron microscopy. In ord germaria, meiotic double strand breaks appear and disappear with frequency and timing indistinguishable from wild type. However, Ring chromosome recovery is dramatically reduced in ord oocytes compared with wild type, which is consistent with the model that defects in meiotic cohesion remove the constraints that normally limit recombination between sisters. We conclude that ORD activity suppresses sister chromatid exchange and stimulates inter-homologue crossovers, thereby promoting homologue bias during meiotic recombination in Drosophila.

Key Words: chromosome segregation; meiosis; sister chromatid cohesion; Drosophila; synaptonemal complex

Abbreviations used in this paper: AE/LE, axial/lateral element; CE, central element; DSB, double strand break; {gamma}-H2Av, phosphorylated H2Av; ord, orientation disruptor; SC, synaptonemal complex.


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