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Published 29 March 2004. doi:10.1083/jcb.200311023
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 7, 1033-1044
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Article

 Conventional PKCs regulate the temporal pattern of Ca2+ oscillations at fertilization in mouse eggs

Guillaume Halet1, Richard Tunwell1, Scott J. Parkinson2, and John Carroll1

1 Department of Physiology, University College London, London WC1E 6BT, England, UK
2 Dana-Farber Cancer Institute, Boston, MA 02115

Address correspondence to Guillaume Halet, Department of Physiology, University College London, Gower Street, London WC1E 6BT, England, UK. Tel.: 0207-679-3229. Fax: 0207-383-7005. email: g.halet{at}ucl.ac.uk; or John Carroll, Department of Physiology, University College London, Gower Street, London WC1E 6BT, England, UK. Tel.: 0207-679-3229. Fax: 0207-383-7005. email: j.carroll{at}ucl.ac.uk

In mammalian eggs, sperm-induced Ca2+ oscillations at fertilization are the primary trigger for egg activation and initiation of embryonic development. Identifying the downstream effectors that decode this unique Ca2+ signal is essential to understand how the transition from egg to embryo is coordinated. Here, we investigated whether conventional PKCs (cPKCs) can decode Ca2+ oscillations at fertilization. By monitoring the dynamics of GFP-labeled PKC{alpha} and PKC{gamma} in living mouse eggs, we demonstrate that cPKCs translocate to the egg membrane at fertilization following a pattern that is shaped by the amplitude, duration, and frequency of the Ca2+ transients. In addition, we show that cPKC translocation is driven by the C2 domain when Ca2+ concentration reaches 1–3 µM. Finally, we present evidence that one physiological function of activated cPKCs in fertilized eggs is to sustain long-lasting Ca2+ oscillations, presumably via the regulation of store-operated Ca2+ entry.

Key Words: calcium; PKC; oscillations; GFP; influx

The online version of this article includes supplemental material.

Abbreviations used in this paper: BIM, bisindolylmaleimide I; cPKC, conventional protein kinase C; DiC8, 1,2-dioctanoyl sn-glycerol; InsP3, inositol 1,4,5-trisphosphate; PIP2, phosphatidylinositol 4,5-bisphosphate; SOC, store-operated channel; SOCE, store-operated calcium entry.


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