Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer

doi:10.1083/jcb.200312034

Published 12 April 2004. doi:10.1083/jcb.200312034
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 165, Number 1, 111-122

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Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer

Matthew N.J. Seaman

Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Cambridge CB2 2XY, England, UK

Address correspondence to M.N.J. Seaman, Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust/MRC Building, Addenbrookes Hospital, Hills Road, Cambridge CB2 2XY, England, UK. Tel.: (44) 1223-762627. Fax: (44) 1223-762640. email: mnjs100{at}cam.ac.uk

fEndosome-to-Golgi retrieval of the mannose 6-phosphate receptor(MPR) is required for lysosome biogenesis. Currently, this pathwayis poorly understood. Analyses in yeast identified a complexof proteins called "retromer" that is essential for endosome-to-Golgiretrieval of the carboxypeptidase Y receptor Vps10p. Retromercomprises five distinct proteins: Vps35p, 29p, 26p, 17p, and5p, which are conserved in mammals. Here, we show that retromeris required for the efficient retrieval of the cation-independentMPR (CI-MPR). Cells lacking mammalian VPS26 fail to retrievethe CI-MPR, resulting in either rapid degradation of or mislocalizationto the plasma membrane. We have localized mVPS26 to multivesicularbody endosomes by electron microscopy, and through the use ofCD8 reporter protein constructs have examined the effect ofloss of mVPS26 upon the trafficking of membrane proteins thatcycle between the endosome and the Golgi. The data presentedhere support the hypothesis that retromer performs a selectivefunction in endosome-to-Golgi transport, mediating retrievalof the CI-MPR, but not furin.

Key Words: retromer; vesicle; retrieval; endosome; Golgi

The online version of this article includes supplemental material.

Abbreviations used in this paper: CD-MPR, cation-dependent mannose6-phosphate receptor; CI-MPR, cation-independent mannose 6-phosphatereceptor; CPY, carboxypeptidase Y; MPR, mannose 6-phosphatereceptor; siRNA, small interfering RNA; Snx, sorting nexin;TfnR, transferrin receptor.

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