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Published online 5 April 2004. doi:10.1083/jcb.200309089
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 165, Number 1, 99-109
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Article

 Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p

Dina Matheos1, Metodi Metodiev2, Eric Muller1, David Stone2, and Mark D. Rose1

1 Department of Molecular Biology, Princeton University, Princeton, NJ 08544
2 Department of Biological Sciences, University of Illinois-Chicago, Chicago, IL 60607

Address correspondence to Mark Rose, Dept. of Molecular Biology, Princeton University, Princeton, NJ 08544-1014. Tel.: (609) 258-2804. Fax: (609) 258-1975. email: mrose{at}molbio.princeton.edu

During mating, budding yeast cells reorient growth toward the highest concentration of pheromone. Bni1p, a formin homologue, is required for this polarized growth by facilitating cortical actin cable assembly. Fus3p, a pheromone-activated MAP kinase, is required for pheromone signaling and cell fusion. We show that Fus3p phosphorylates Bni1p in vitro, and phosphorylation of Bni1p in vivo during the pheromone response is dependent on Fus3p. fus3 mutants exhibited multiple phenotypes similar to bni1 mutants, including defects in actin and cell polarization, as well as Kar9p and cytoplasmic microtubule localization. Disruption of the interaction between Fus3p and the receptor-associated G{alpha} subunit caused similar mutant phenotypes. After pheromone treatment, Bni1p-GFP and Spa2p failed to localize to the cortex of fus3 mutants, and cell wall growth became completely unpolarized. Bni1p overexpression suppressed the actin assembly, cell polarization, and cell fusion defects. These data suggest a model wherein activated Fus3p is recruited back to the cortex, where it activates Bni1p to promote polarization and cell fusion.

Key Words: yeast; mating; actin; cytoskeleton; signal transduction

Abbreviation used in this paper: 1-Na PP1, 1-napthyl PP1.


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