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Published 10 May 2004. doi:10.1083/jcb.200311076
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 165, Number 3, 313-321
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Article

 Golgi duplication in Trypanosoma brucei

Cynthia Y. He1, Helen H. Ho1, Joerg Malsam1, Cecile Chalouni1, Christopher M. West3, Elisabetta Ullu2, Derek Toomre1, and Graham Warren1

1 Department of Cell Biology, Ludwig Institute for Cancer Research
2 Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520
3 Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104

Address correspondence to Graham Warren, Dept. of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar St., P.O. Box 208002, New Haven, CT 06520-8002. Tel.: (203) 785-5058. Fax: (203) 785-4301. email: graham.warren{at}yale.edu

Duplication of the single Golgi apparatus in the protozoan parasite Trypanosoma brucei has been followed by tagging a putative Golgi enzyme and a matrix protein with variants of GFP. Video microscopy shows that the new Golgi appears de novo, near to the old Golgi, about two hours into the cell cycle and grows over a two-hour period until it is the same size as the old Golgi. Duplication of the endoplasmic reticulum (ER) export site follows exactly the same time course. Photobleaching experiments show that the new Golgi is not the exclusive product of the new ER export site. Rather, it is supplied, at least in part, by material directly from the old Golgi. Pharmacological experiments show that the site of the new Golgi and ER export is determined by the location of the new basal body.

Key Words: ER export site; fluorescence recovery after photobleaching; GRASP; organelle biogenesis; basal body

The online version of this article includes supplemental material.

Abbreviation used in this paper: CGN, cis Golgi network.


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