Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 6470K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Aguirre, A. A. Articles by Gallo, V. Search for Related Content PubMed PubMed Citation Articles by Aguirre, A. A. Articles by Gallo, V. Related Collections Related Article Social Bookmarking                      What's this?

¹ Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center, Washington, DC 20010
² Center for Cellular and Molecular Neurobiology, University of Liège, 4020 Liège, Belgium

Address correspondence to Vittorio Gallo, Center for Neuroscience Research, Children's Research Institute, Rm. 5345, Children's National Medical Center, 111 Michigan Ave., N.W., Washington, DC 20010. Tel.: (202) 884-4996. Fax: (202) 884-4988. email: vgallo{at}cnmcresearch.org

The subventricular zone (SVZ) is a source of neural progenitors throughout brain development. The identification and purification of these progenitors and the analysis of their lineage potential are fundamental issues for future brain repair therapies. We demonstrate that early postnatal NG2-expressing (NG2⁺) progenitor cells located in the SVZ self-renew in vitro and display phenotypic features of transit-amplifier type C–like multipotent cells. NG2⁺ cells in the SVZ are highly proliferative and express the epidermal growth factor receptor, the transcription factors Dlx, Mash1, and Olig2, and the Lewis X (LeX) antigen. We show that grafted early postnatal NG2⁺ cells generate hippocampal GABAergic interneurons that propagate action potentials and receive functional glutamatergic synaptic inputs. Our work identifies Dlx⁺/Mash1⁺/LeX⁺/NG2⁺/GFAP-negative cells of the SVZ as a new class of postnatal multipotent progenitor cells that may represent a specific cellular reservoir for renewal of postnatal and adult inhibitory interneurons in the hippocampus.

Key Words: neural stem cells; homeobox transcription factors; bHLH genes; progenitor cell transplantation; synaptic transmission

Abbreviations used in this paper: CNP, 2',3'-cyclic nucleotide 3'-phosphodiesterase; DG, dentate gyrus; DIV, days in vitro; EGFR, epidermal growth factor receptor; LeX, Lewis X; NSC, neural stem cell; SVZ, subventricular zone.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

NG2⁺ cells feed the brain

Nicole LeBrasseur
J. Cell Biol. 2004 165: 455. [Full Text] [PDF]

This article has been cited by other articles:

• Tanaka, Y., Tozuka, Y., Takata, T., Shimazu, N., Matsumura, N., Ohta, A., Hisatsune, T. (2009). Excitatory GABAergic Activation of Cortical Dividing Glial Cells. Cereb Cortex 0: bhn238v1-bhn238 [Abstract] [Full Text]  
• Dimou, L., Simon, C., Kirchhoff, F., Takebayashi, H., Gotz, M. (2008). Progeny of Olig2-Expressing Progenitors in the Gray and White Matter of the Adult Mouse Cerebral Cortex. J. Neurosci. 28: 10434-10442 [Abstract] [Full Text]  
• Hisahara, S., Chiba, S., Matsumoto, H., Tanno, M., Yagi, H., Shimohama, S., Sato, M., Horio, Y. (2008). From the Cover: Histone deacetylase SIRT1 modulates neuronal differentiation by its nuclear translocation. Proc. Natl. Acad. Sci. USA 105: 15599-15604 [Abstract] [Full Text]  
• Bonaguidi, M. A., Peng, C.-Y., McGuire, T., Falciglia, G., Gobeske, K. T., Czeisler, C., Kessler, J. A. (2008). Noggin Expands Neural Stem Cells in the Adult Hippocampus. J. Neurosci. 28: 9194-9204 [Abstract] [Full Text]  
• Chang, A., Smith, M. C., Yin, X., Fox, R. J., Staugaitis, S. M., Trapp, B. D. (2008). Neurogenesis in the chronic lesions of multiple sclerosis. Brain 131: 2366-2375 [Abstract] [Full Text]  
• Gallo, V., Mangin, J.-M., Kukley, M., Dietrich, D. (2008). Synapses on NG2-expressing progenitors in the brain: multiple functions?. J. Physiol. 586: 3767-3781 [Abstract] [Full Text]  
• Mangin, J.-M., Kunze, A., Chittajallu, R., Gallo, V. (2008). Satellite NG2 Progenitor Cells Share Common Glutamatergic Inputs with Associated Interneurons in the Mouse Dentate Gyrus. J. Neurosci. 28: 7610-7623 [Abstract] [Full Text]  
• Dubois-Dalcq, M., Williams, A., Stadelmann, C., Stankoff, B., Zalc, B., Lubetzki, C. (2008). From fish to man: understanding endogenous remyelination in central nervous system demyelinating diseases. Brain 131: 1686-1700 [Abstract] [Full Text]  
• Katsimpardi, L., Gaitanou, M., Malnou, C. E., Lledo, P.-M., Charneau, P., Matsas, R., Thomaidou, D. (2008). BM88/Cend1 Expression Levels Are Critical for Proliferation and Differentiation of Subventricular Zone-Derived Neural Precursor Cells. Stem Cells 26: 1796-1807 [Abstract] [Full Text]  
• Zhu, X., Bergles, D. E., Nishiyama, A. (2008). NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development 135: 145-157 [Abstract] [Full Text]  
• Jablonska, B., Aguirre, A., Vandenbosch, R., Belachew, S., Berthet, C., Kaldis, P., Gallo, V. (2007). Cdk2 is critical for proliferation and self-renewal of neural progenitor cells in the adult subventricular zone. JCB 179: 1231-1245 [Abstract] [Full Text]  
• Mandyam, C. D., Wee, S., Eisch, A. J., Richardson, H. N., Koob, G. F. (2007). Methamphetamine Self-Administration and Voluntary Exercise Have Opposing Effects on Medial Prefrontal Cortex Gliogenesis. J. Neurosci. 27: 11442-11450 [Abstract] [Full Text]  
• Chittajallu, R., Kunze, A., Mangin, J.-M., Gallo, V. (2007). Differential Synaptic Integration of Interneurons in the Outer and Inner Molecular Layers of the Developing Dentate Gyrus. J. Neurosci. 27: 8219-8225 [Abstract] [Full Text]  
• Nakano, I., Dougherty, J. D., Kim, K., Klement, I., Geschwind, D. H., Kornblum, H. I. (2007). Phosphoserine Phosphatase Is Expressed in the Neural Stem Cell Niche and Regulates Neural Stem and Progenitor Cell Proliferation. Stem Cells 25: 1975-1984 [Abstract] [Full Text]  
• Yanagisawa, M., Yu, R. K (2007). The expression and functions of glycoconjugates in neural stem cells. Glycobiology 17: 57R-74R [Abstract] [Full Text]  
• Nishiyama, A. (2007). Polydendrocytes: NG2 Cells with Many Roles in Development and Repair of the CNS. Neuroscientist 13: 62-76 [Abstract]  
• Dromard, C., Bartolami, S., Deleyrolle, L., Takebayashi, H., Ripoll, C., Simonneau, L., Prome, S., Puech, S., Tran Van Ba, C., Duperray, C., Valmier, J., Privat, A., Hugnot, J.-P. (2007). NG2 and Olig2 Expression Provides Evidence for Phenotypic Deregulation of Cultured Central Nervous System and Peripheral Nervous System Neural Precursor Cells. Stem Cells 25: 340-353 [Abstract] [Full Text]  
• Kudo, T., Fujii, T., Ikegami, S., Inokuchi, K., Takayama, Y., Ikehara, Y., Nishihara, S., Togayachi, A., Takahashi, S., Tachibana, K., Yuasa, S., Narimatsu, H. (2007). Mice lacking {alpha}1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors. Glycobiology 17: 1-9 [Abstract] [Full Text]  
• Ganat, Y. M., Silbereis, J., Cave, C., Ngu, H., Anderson, G. M., Ohkubo, Y., Ment, L. R., Vaccarino, F. M. (2006). Early Postnatal Astroglial Cells Produce Multilineage Precursors and Neural Stem Cells In Vivo.. J. Neurosci. 26: 8609-8621 [Abstract] [Full Text]  
• Menn, B., Garcia-Verdugo, J. M., Yaschine, C., Gonzalez-Perez, O., Rowitch, D., Alvarez-Buylla, A. (2006). Origin of oligodendrocytes in the subventricular zone of the adult brain.. J. Neurosci. 26: 7907-7918 [Abstract] [Full Text]  
• Ligon, K. L., Kesari, S., Kitada, M., Sun, T., Arnett, H. A., Alberta, J. A., Anderson, D. J., Stiles, C. D., Rowitch, D. H. (2006). Development of NG2 neural progenitor cells requires Olig gene function. Proc. Natl. Acad. Sci. USA 103: 7853-7858 [Abstract] [Full Text]  
• von Holst, A., Sirko, S., Faissner, A. (2006). The Unique 473HD-Chondroitinsulfate Epitope Is Expressed by Radial Glia and Involved in Neural Precursor Cell Proliferation. J. Neurosci. 26: 4082-4094 [Abstract] [Full Text]  
• Gal, J. S., Morozov, Y. M., Ayoub, A. E., Chatterjee, M., Rakic, P., Haydar, T. F. (2006). Molecular and Morphological Heterogeneity of Neural Precursors in the Mouse Neocortical Proliferative Zones. J. Neurosci. 26: 1045-1056 [Abstract] [Full Text]  
• Buffo, A., Vosko, M. R., Erturk, D., Hamann, G. F., Jucker, M., Rowitch, D., Gotz, M. (2005). Expression pattern of the transcription factor Olig2 in response to brain injuries: Implications for neuronal repair. Proc. Natl. Acad. Sci. USA 102: 18183-18188 [Abstract] [Full Text]  
• Aguirre, A., Rizvi, T. A., Ratner, N., Gallo, V. (2005). Overexpression of the Epidermal Growth Factor Receptor Confers Migratory Properties to Nonmigratory Postnatal Neural Progenitors. J. Neurosci. 25: 11092-11106 [Abstract] [Full Text]  
• Dougherty, J. D., Garcia, A. D. R., Nakano, I., Livingstone, M., Norris, B., Polakiewicz, R., Wexler, E. M., Sofroniew, M. V., Kornblum, H. I., Geschwind, D. H. (2005). PBK/TOPK, a Proliferating Neural Progenitor-Specific Mitogen-Activated Protein Kinase Kinase. J. Neurosci. 25: 10773-10785 [Abstract] [Full Text]  
• Chittajallu, R., Aguirre, A. A., Gallo, V. (2005). Downregulation of Platelet-Derived Growth Factor-{alpha} Receptor-Mediated Tyrosine Kinase Activity as a Cellular Mechanism for K+-Channel Regulation during Oligodendrocyte Development In Situ. J. Neurosci. 25: 8601-8610 [Abstract] [Full Text]  
• Jabs, R., Pivneva, T., Huttmann, K., Wyczynski, A., Nolte, C., Kettenmann, H., Steinhauser, C. (2005). Synaptic transmission onto hippocampal glial cells with hGFAP promoter activity. J. Cell Sci. 118: 3791-3803 [Abstract] [Full Text]  
• Dayer, A. G., Cleaver, K. M., Abouantoun, T., Cameron, H. A. (2005). New GABAergic interneurons in the adult neocortex and striatum are generated from different precursors. JCB 168: 415-427 [Abstract] [Full Text]  
• Chojnacki, A., Weiss, S. (2004). Isolation of a Novel Platelet-Derived Growth Factor-Responsive Precursor from the Embryonic Ventral Forebrain. J. Neurosci. 24: 10888-10899 [Abstract] [Full Text]  
• Aguirre, A., Gallo, V. (2004). Postnatal Neurogenesis and Gliogenesis in the Olfactory Bulb from NG2-Expressing Progenitors of the Subventricular Zone. J. Neurosci. 24: 10530-10541 [Abstract] [Full Text]  
• Chittajallu, R, Aguirre, A, Gallo, V (2004). NG2-positive cells in the mouse white and grey matter display distinct physiological properties. J. Physiol. 561: 109-122 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents