NG2-expressing cells in the subventricular zone are type C-like cells and contribute to interneuron generation in the postnatal hippocampus

doi:10.1083/jcb.200311141

Published 24 May 2004. doi:10.1083/jcb.200311141
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 165, Number 4, 575-589

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NG2-expressing cells in the subventricular zone are type C–like cells and contribute to interneuron generation in the postnatal hippocampus

Adan A. Aguirre¹, Ramesh Chittajallu¹, Shibeshih Belachew², and Vittorio Gallo¹

¹ Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center, Washington, DC 20010
² Center for Cellular and Molecular Neurobiology, University of Liège, 4020 Liège, Belgium

Address correspondence to Vittorio Gallo, Center for Neuroscience Research, Children's Research Institute, Rm. 5345, Children's National Medical Center, 111 Michigan Ave., N.W., Washington, DC 20010. Tel.: (202) 884-4996. Fax: (202) 884-4988. email: vgallo{at}cnmcresearch.org

The subventricular zone (SVZ) is a source of neural progenitorsthroughout brain development. The identification and purificationof these progenitors and the analysis of their lineage potentialare fundamental issues for future brain repair therapies. Wedemonstrate that early postnatal NG2-expressing (NG2⁺) progenitorcells located in the SVZ self-renew in vitro and display phenotypicfeatures of transit-amplifier type C–like multipotentcells. NG2⁺ cells in the SVZ are highly proliferative and expressthe epidermal growth factor receptor, the transcription factorsDlx, Mash1, and Olig2, and the Lewis X (LeX) antigen. We showthat grafted early postnatal NG2⁺ cells generate hippocampalGABAergic interneurons that propagate action potentials andreceive functional glutamatergic synaptic inputs. Our work identifiesDlx⁺/Mash1⁺/LeX⁺/NG2⁺/GFAP-negative cells of the SVZ as a newclass of postnatal multipotent progenitor cells that may representa specific cellular reservoir for renewal of postnatal and adultinhibitory interneurons in the hippocampus.

Key Words: neural stem cells; homeobox transcription factors; bHLH genes; progenitor cell transplantation; synaptic transmission

The online version of this article includes supplemental material.

Abbreviations used in this paper: CNP, 2',3'-cyclic nucleotide3'-phosphodiesterase; DG, dentate gyrus; DIV, days in vitro;EGFR, epidermal growth factor receptor; LeX, Lewis X; NSC, neuralstem cell; SVZ, subventricular zone.

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