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Published online 14 June 2004. doi:10.1083/jcb.200311054
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 165, Number 6, 813-822
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Article

Annexin 11 is required for midbody formation and completion of the terminal phase of cytokinesis



Alejandra Tomas, Clare Futter, and Stephen E. Moss

Division of Cell Biology, Institute of Ophthalmology, London EC1V 9EL, England, UK

Address correspondence to S.E. Moss, Division of Cell Biology, Institute of Ophthalmology, 11-43 Bath St., London EC1V 9EL, England, UK. Tel.: 020 7608 6973. Fax: 020 7608 4034. email: s.moss{at}ucl.ac.uk

Annexins are Ca2+-binding, membrane-fusogenic proteins with diverse but poorly understood functions. Here, we show that during cell cycle progression annexin 11 translocates from the nucleus to the spindle poles in metaphase and to the spindle midzone in anaphase. Annexin 11 is recruited to the midbody in late telophase, where it forms part of the detergent-resistant matrix that also contains CHO1. To investigate the significance of these observations, we used RNA interference to deplete cells of annexin 11. A combination of confocal and video time-lapse microscopy revealed that cells lacking annexin 11 fail to establish a functional midbody. Instead, daughter cells remain connected by intercellular bridges that contain bundled microtubules and cytoplasmic organelles but exclude normal midbody components such as MKLP1 and Aurora B. Annexin 11–depleted cells failed to complete cytokinesis and died by apoptosis. These findings demonstrate an essential role for annexin 11 in the terminal phase of cytokinesis.

Key Words: calcium; mitosis; telophase; cell cycle; kinesin

The online version of this article includes supplemental material.

Abbreviations used in this paper: BFA, brefeldin A; RNAi, RNA interference.


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