JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 5 July 2004. doi:10.1083/jcb.200310145
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 166, Number 1, 111-119
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1620K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Caroppo, R.
Right arrow Articles by Curci, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caroppo, R.
Right arrow Articles by Curci, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Extracellular calcium acts as a "third messenger" to regulate enzyme and alkaline secretion

Rosa Caroppo1, Andrea Gerbino1, Gregorio Fistetto1, Matilde Colella1,2, Lucantonio Debellis1, Aldebaran M. Hofer2, and Silvana Curci1,2

1 Dipartimento di Fisiologia Generale ed Ambientale, Università di Bari, 70126 Bari, Italy
2 Department of Surgery, Harvard Medical School, Brigham and Women's Hospital and the Boston VA Healthcare System, West Roxbury, MA 02132

Address correspondence to S. Curci, Dept. of Surgery, Harvard Medical School, Brigham and Women's Hospital and the Boston VA Healthcare System, 1400 VFW Parkway, West Roxbury, MA 02132. Tel.: (617) 323-7700, ext. 5902. Fax: (617) 363-5592. email: scurci{at}rics.bwh.harvard.edu


Abstract
It is generally assumed that the functional consequences of stimulation with Ca2+-mobilizing agonists are derived exclusively from the second messenger action of intracellular Ca2+, acting on targets inside the cells. However, during Ca2+ signaling events, Ca2+ moves in and out of the cell, causing changes not only in intracellular Ca2+, but also in local extracellular Ca2+. The fact that numerous cell types possess an extracellular Ca2+ "sensor" raises the question of whether these dynamic changes in external [Ca2+] may serve some sort of messenger function. We found that in intact gastric mucosa, the changes in extracellular [Ca2+] secondary to carbachol-induced increases in intracellular [Ca2+] were sufficient and necessary to elicit alkaline secretion and pepsinogen secretion, independent of intracellular [Ca2+] changes. These findings suggest that extracellular Ca2+ can act as a "third messenger" via Ca2+ sensor(s) to regulate specific subsets of tissue function previously assumed to be under the direct control of intracellular Ca2+.

Key Words: extracellular Ca2+-sensing receptor; polarized epithelial cells; pH microelectrodes; interstitial space; adenylyl cyclase

Abbreviations used in this paper: AC, adenylyl cyclase; CaR, Ca2+-sensing receptor; DIDS, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid; OC, oxyntopeptic cell; PMCA, plasma membrane Ca2+ ATPase; PTX, pertussis toxin; SQ 22,536, 9-(tetrahydro-2-furanyl)-9H-purin-6-amine; tBHQ, 2,5-di-(tert-butyl)hydroquinone.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents