Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 3253K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Blache, P. Articles by Jay, P. Search for Related Content PubMed PubMed Citation Articles by Blache, P. Articles by Jay, P. Pubmed/NCBI databases Gene GEO Profiles HomoloGene OMIM UniGene Compound via MeSH Substance via MeSH Genetics Home Reference Social Bookmarking                            What's this?

SOX9 is an intestine crypt transcription factor, is regulated by the Wnt pathway, and represses the CDX2 and MUC2 genes

¹ Institut de Génétique Humaine, Centre National de la Recherche Scientifique (CNRS) UPR1142, 34396 Montpellier, Cedex 5, France
² Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, Netherlands
³ Institut National de la Santé et de la Recherche Médicale (INSERM) U381, F-67200 Strasbourg, France

Address correspondence to Philippe Jay, Institut de Génétique Humaine, CNRS UPR1142, 141 rue de la Cardonille, 34396 Montpellier, Cedex 5, France. Tel.: 33-499-61-99-40. Fax: 33-499-61-99-01. email: pj{at}igh.cnrs.fr

Abstract

TCF and SOX proteins belong to the high mobility group box transcription factor family. Whereas TCFs, the transcriptional effectors of the Wnt pathway, have been widely implicated in the development, homeostasis and disease of the intestine epithelium, little is known about the function of the SOX proteins in this tissue. Here, we identified SOX9 in a SOX expression screening in the mouse fetal intestine. We report that the SOX9 protein is expressed in the intestinal epithelium in a pattern characteristic of Wnt targets. We provide in vitro and in vivo evidence that a bipartite ß-catenin/TCF4 transcription factor, the effector of the Wnt signaling pathway, is required for SOX9 expression in epithelial cells. Finally, in colon epithelium-derived cells, SOX9 transcriptionally represses the CDX2 and MUC2 genes, normally expressed in the mature villus cells of the intestinal epithelium, and may therefore contribute to the Wnt-dependent maintenance of a progenitor cell phenotype.

Key Words: SOX9; Wnt; CDX2; intestinal epithelium; differentiation

Abbreviations used in this paper: CD, campomelic dysplasia; cyt-E-cadherin, cytoplasmic domain of E-cadherin.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Carpentino, J. E., Hynes, M. J., Appelman, H. D., Zheng, T., Steindler, D. A., Scott, E. W., Huang, E. H. (2009). Aldehyde Dehydrogenase-Expressing Colon Stem Cells Contribute to Tumorigenesis in the Transition from Colitis to Cancer. Cancer Res. 69: 8208-8215 [Abstract] [Full Text]  
• Dupasquier, S., Abdel-Samad, R., Glazer, R. I., Bastide, P., Jay, P., Joubert, D., Cavailles, V., Blache, P., Quittau-Prevostel, C. (2009). A new mechanism of SOX9 action to regulate PKC{alpha} expression in the intestine epithelium. J. Cell Sci. 122: 2191-2196 [Abstract] [Full Text]  
• Formeister, E. J., Sionas, A. L., Lorance, D. K., Barkley, C. L., Lee, G. H., Magness, S. T. (2009). Distinct SOX9 levels differentially mark stem/progenitor populations and enteroendocrine cells of the small intestine epithelium. Am. J. Physiol. Gastrointest. Liver Physiol. 296: G1108-G1118 [Abstract] [Full Text]  
• Rodilla, V., Villanueva, A., Obrador-Hevia, A., Robert-Moreno, A., Fernandez-Majada, V., Grilli, A., Lopez-Bigas, N., Bellora, N., Alba, M. M., Torres, F., Dunach, M., Sanjuan, X., Gonzalez, S., Gridley, T., Capella, G., Bigas, A., Espinosa, L. (2009). Jagged1 is the pathological link between Wnt and Notch pathways in colorectal cancer. Proc. Natl. Acad. Sci. USA 106: 6315-6320 [Abstract] [Full Text]  
• Dichtel-Danjoy, M.-L., Caldeira, J., Casares, F. (2009). SoxF is part of a novel negative-feedback loop in the wingless pathway that controls proliferation in the Drosophila wing disc. Development 136: 761-769 [Abstract] [Full Text]  
• Topol, L., Chen, W., Song, H., Day, T. F., Yang, Y. (2009). Sox9 Inhibits Wnt Signaling by Promoting {beta}-Catenin Phosphorylation in the Nucleus. J. Biol. Chem. 284: 3323-3333 [Abstract] [Full Text]  
• Yang, K., Popova, N. V., Yang, W. C., Lozonschi, I., Tadesse, S., Kent, S., Bancroft, L., Matise, I., Cormier, R. T., Scherer, S. J., Edelmann, W., Lipkin, M., Augenlicht, L., Velcich, A. (2008). Interaction of Muc2 and Apc on Wnt Signaling and in Intestinal Tumorigenesis: Potential Role of Chronic Inflammation. Cancer Res. 68: 7313-7322 [Abstract] [Full Text]  
• Yamaguchi, K., Cekanova, M., McEntee, M. F., Yoon, J.-H., Fischer, S. M., Renes, I. B., Van Seuningen, I., Baek, S. J. (2008). Peroxisome proliferator-activated receptor ligand MCC-555 suppresses intestinal polyps in ApcMin/+ mice via extracellular signal-regulated kinase and peroxisome proliferator-activated receptor-dependent pathways. Molecular Cancer Therapeutics 7: 2779-2787 [Abstract] [Full Text]  
• Mathew, L. K., Sengupta, S. S., LaDu, J., Andreasen, E. A., Tanguay, R. L. (2008). Crosstalk between AHR and Wnt signaling through R-Spondin1 impairs tissue regeneration in zebrafish. FASEB J. 22: 3087-3096 [Abstract] [Full Text]  
• Darido, C., Buchert, M., Pannequin, J., Bastide, P., Zalzali, H., Mantamadiotis, T., Bourgaux, J.-F., Garambois, V., Jay, P., Blache, P., Joubert, D., Hollande, F. (2008). Defective Claudin-7 Regulation by Tcf-4 and Sox-9 Disrupts the Polarity and Increases the Tumorigenicity of Colorectal Cancer Cells. Cancer Res. 68: 4258-4268 [Abstract] [Full Text]  
• Wang, H., Leav, I., Ibaragi, S., Wegner, M., Hu, G.-f., Lu, M. L., Balk, S. P., Yuan, X. (2008). SOX9 Is Expressed in Human Fetal Prostate Epithelium and Enhances Prostate Cancer Invasion. Cancer Res. 68: 1625-1630 [Abstract] [Full Text]  
• de Bont, J. M., Kros, J. M., Passier, M. M.C.J., Reddingius, R. E., Smitt, P. A.E. S., Luider, T. M., Boer, M. L. d., Pieters, R. (2008). Differential expression and prognostic significance of SOX genes in pediatric medulloblastoma and ependymoma identified by microarray analysis. Neuro Oncol Duke 10: 648-660 [Abstract] [Full Text]  
• Dube, P. E., Rowland, K. J., Brubaker, P. L. (2008). Glucagon-Like Peptide-2 Activates -Catenin Signaling in the Mouse Intestinal Crypt: Role of Insulin-Like Growth Factor-I. Endocrinology 149: 291-301 [Abstract] [Full Text]  
• Ramocki, N. M., Wilkins, H. R., Magness, S. T., Simmons, J. G., Scull, B. P., Lee, G. H., McNaughton, K. K., Lund, P. K. (2008). Insulin Receptor Substrate-1 Deficiency Promotes Apoptosis in the Putative Intestinal Crypt Stem Cell Region, Limits Apcmin/+ Tumors, and Regulates Sox9. Endocrinology 149: 261-267 [Abstract] [Full Text]  
• Gulati, A. S., Ochsner, S. A., Henning, S. J. (2008). Molecular properties of side population-sorted cells from mouse small intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 294: G286-G294 [Abstract] [Full Text]  
• Saaf, A. M., Halbleib, J. M., Chen, X., Yuen, S. T., Leung, S. Y., Nelson, W. J., Brown, P. O. (2007). Parallels between Global Transcriptional Programs of Polarizing Caco-2 Intestinal Epithelial Cells In Vitro and Gene Expression Programs in Normal Colon and Colon Cancer. Mol. Biol. Cell 18: 4245-4260 [Abstract] [Full Text]  
• Bastide, P., Darido, C., Pannequin, J., Kist, R., Robine, S., Marty-Double, C., Bibeau, F., Scherer, G., Joubert, D., Hollande, F., Blache, P., Jay, P. (2007). Sox9 regulates cell proliferation and is required for Paneth cell differentiation in the intestinal epithelium. JCB 178: 635-648 [Abstract] [Full Text]  
• Schneikert, J., Behrens, J. (2007). The canonical Wnt signalling pathway and its APC partner in colon cancer development. Gut 56: 417-425 [Full Text]  
• Seymour, P. A., Freude, K. K., Tran, M. N., Mayes, E. E., Jensen, J., Kist, R., Scherer, G., Sander, M. (2007). From the Cover: SOX9 is required for maintenance of the pancreatic progenitor cell pool. Proc. Natl. Acad. Sci. USA 104: 1865-1870 [Abstract] [Full Text]  
• Benahmed, F., Gross, I., Guenot, D., Jehan, F., Martin, E., Domon-Dell, C., Brabletz, T., Kedinger, M., Freund, J.-N., Duluc, I. (2007). The Microenvironment Controls CDX2 Homeobox Gene Expression in Colorectal Cancer Cells. Am. J. Pathol. 170: 733-744 [Abstract] [Full Text]  
• Wang, H., McKnight, N. C., Zhang, T., Lu, M. L., Balk, S. P., Yuan, X. (2007). SOX9 Is Expressed in Normal Prostate Basal Cells and Regulates Androgen Receptor Expression in Prostate Cancer Cells. Cancer Res. 67: 528-536 [Abstract] [Full Text]  
• Calon, A., Gross, I., Davidson, I., Kedinger, M., Duluc, I., Domon-Dell, C., Freund, J.-N. (2007). Functional interaction between the homeoprotein CDX1 and the transcriptional machinery containing the TATA-binding protein. Nucleic Acids Res 35: 175-185 [Abstract] [Full Text]  
• Kim, S., Xu, X., Hecht, A., Boyer, T. G. (2006). Mediator Is a Transducer of Wnt/beta-Catenin Signaling. J. Biol. Chem. 281: 14066-14075 [Abstract] [Full Text]  
• Wilson, M. E., Yang, K. Y., Kalousova, A., Lau, J., Kosaka, Y., Lynn, F. C., Wang, J., Mrejen, C., Episkopou, V., Clevers, H. C., German, M. S. (2005). The HMG Box Transcription Factor Sox4 Contributes to the Development of the Endocrine Pancreas. Diabetes 54: 3402-3409 [Abstract] [Full Text]  
• Kleber, M., Lee, H.-Y., Wurdak, H., Buchstaller, J., Riccomagno, M. M., Ittner, L. M., Suter, U., Epstein, D. J., Sommer, L. (2005). Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling. JCB 169: 309-320 [Abstract] [Full Text]  
• Jay, P., Berta, P., Blache, P. (2005). Expression of the Carcinoembryonic Antigen Gene Is Inhibited by SOX9 in Human Colon Carcinoma Cells. Cancer Res. 65: 2193-2198 [Abstract] [Full Text]  
• Okubo, T., Knoepfler, P. S., Eisenman, R. N., Hogan, B. L. M. (2005). Nmyc plays an essential role during lung development as a dosage-sensitive regulator of progenitor cell proliferation and differentiation. Development 132: 1363-1374 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents