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Published 19 July 2004. doi:10.1083/jcb.200403084
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 166, Number 2, 167-172
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Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2



Ulrike Gruneberg2, Rüdiger Neef1, Reiko Honda2, Erich A. Nigg2, and Francis A. Barr1

1 Intracellular Protein Transport, Independent Junior Research Group
2 Department of Cell Biology, Max-Planck-Institute of Biochemistry, Martinsried, 82152 Germany

Address correspondence to Francis Barr, Intracellular Protein Transport, Independent Junior Research Group, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, Martinsried, 82152 Germany. Tel.: 49-89-8578-3135. Fax: 49-89-8578-3102. email: barr{at}biochem.mpg.de

Mitotic kinases of the Polo and Aurora families are key regulators of chromosome segregation and cytokinesis. Here, we have investigated the role of MKlp1 and MKlp2, two vertebrate mitotic kinesins essential for cytokinesis, in the spatial regulation of the Aurora B kinase. Previously, we have demonstrated that MKlp2 recruits Polo-like kinase 1 (Plk1) to the central spindle in anaphase. We now find that in MKlp2 but not MKlp1-depleted cells the Aurora B–INCENP complex remains at the centromeres and fails to relocate to the central spindle. MKlp2 exerts dual control over Aurora B localization, because it is a binding partner for Aurora B, and furthermore for the phosphatase Cdc14A. Cdc14A can dephosphorylate INCENP and may contribute to its relocation to the central spindle in anaphase. We propose that MKlp2 is involved in the localization of Plk1, Aurora B, and Cdc14A to the central spindle during anaphase, and that the integration of signaling by these proteins is necessary for proper cytokinesis.

Key Words: passenger proteins; mitotic kinesins; MKlp1; rabkinesin-6; cytokinesis

Abbreviations used in this paper: MKlp2Ct, MKlp2 stalk-tail domain; MKlp2Nt, MKlp2 lacking the cargo-binding tail domain; Plk1, Polo-like kinase 1.


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