Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 7902K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gassmann, R. Articles by Earnshaw, W. C. Search for Related Content PubMed PubMed Citation Articles by Gassmann, R. Articles by Earnshaw, W. C. Related Collections Related Article Social Bookmarking                            What's this?

Borealin

Reto Gassmann¹, Ana Carvalho^1,4, Alexander J. Henzing¹, Sandrine Ruchaud¹, Damien F. Hudson¹, Reiko Honda³, Erich A. Nigg³, Dietlind L. Gerloff², and William C. Earnshaw¹

¹ Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK
² Institute of Structural and Molecular Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK
³ Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany
⁴ Institute of Molecular Pathology and Immunology of the University of Porto, University of Porto, Porto, Portugal

Address correspondence to W.C. Earnshaw, Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Kings Buildings, Mayfield Rd., Edinburgh EH9 3JR, Scotland, UK. Tel.: 44-131-650-7101. Fax: 44-131-650-7100. email: bill.earnshaw{at}ed.ac.uk

The chromosomal passenger complex of Aurora B kinase, INCENP, and Survivin has essential regulatory roles at centromeres and the central spindle in mitosis. Here, we describe Borealin, a novel member of the complex. Approximately half of Aurora B in mitotic cells is complexed with INCENP, Borealin, and Survivin; and Borealin binds Survivin and INCENP in vitro. A second complex contains Aurora B and INCENP, but no Borealin or Survivin. Depletion of Borealin by RNA interference delays mitotic progression and results in kinetochore–spindle misattachments and an increase in bipolar spindles associated with ectopic asters. The extra poles, which apparently form after chromosomes achieve a bipolar orientation, severely disrupt the partitioning of chromosomes in anaphase. Borealin depletion has little effect on histone H3 serine¹⁰ phosphorylation. These results implicate the chromosomal passenger holocomplex in the maintenance of spindle integrity and suggest that histone H3 serine¹⁰ phosphorylation is performed by an Aurora B–INCENP subcomplex.

Key Words: mitosis; Aurora B kinase; Survivin; INCENP; TD-60

R. Honda's present address is Laboratory of Molecular Biophysics, University of Oxford, South Parks Rd., Oxford OX1 3QU, England, UK.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

Related Article

Another passenger on chromosomes

Nicole LeBrasseur
J. Cell Biol. 2004 166: 152-153. [Full Text] [PDF]

This article has been cited by other articles:

• Liu, J., Wang, Z., Jiang, K., Zhang, L., Zhao, L., Hua, S., Yan, F., Yang, Y., Wang, D., Fu, C., Ding, X., Guo, Z., Yao, X. (2009). PRC1 Cooperates with CLASP1 to Organize Central Spindle Plasticity in Mitosis. J. Biol. Chem. 284: 23059-23071 [Abstract] [Full Text]  
• Bergman, L. M., Birts, C. N., Darley, M., Gabrielli, B., Blaydes, J. P. (2009). CtBPs Promote Cell Survival through the Maintenance of Mitotic Fidelity. Mol. Cell. Biol. 29: 4539-4551 [Abstract] [Full Text]  
• Bohnert, K. A., Chen, J.-S., Clifford, D. M., Vander Kooi, C. W., Gould, K. L. (2009). A Link between Aurora Kinase and Clp1/Cdc14 Regulation Uncovered by the Identification of a Fission Yeast Borealin-Like Protein. Mol. Biol. Cell 20: 3646-3659 [Abstract] [Full Text]  
• Wen, Q., Leung, C., Huang, Z., Small, S., Reddi, A. L., Licht, J. D., Crispino, J. D. (2009). Survivin is not required for the endomitotic cell cycle of megakaryocytes. Blood 114: 153-156 [Abstract] [Full Text]  
• Fu, J., Bian, M., Liu, J., Jiang, Q., Zhang, C. (2009). A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Proc. Natl. Acad. Sci. USA 106: 6939-6944 [Abstract] [Full Text]  
• Nakajima, Y., Tyers, R. G., Wong, C. C.L., Yates, J. R. III, Drubin, D. G., Barnes, G. (2009). Nbl1p: A Borealin/Dasra/CSC-1-like Protein Essential for Aurora/Ipl1 Complex Function and Integrity in Saccharomyces cerevisiae. Mol. Biol. Cell 20: 1772-1784 [Abstract] [Full Text]  
• Klein, U. R., Haindl, M., Nigg, E. A., Muller, S. (2009). RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin. Mol. Biol. Cell 20: 410-418 [Abstract] [Full Text]  
• Woolner, S., O'Brien, L. L., Wiese, C., Bement, W. M. (2008). Myosin-10 and actin filaments are essential for mitotic spindle function. JCB 182: 77-88 [Abstract] [Full Text]  
• Yue, Z., Carvalho, A., Xu, Z., Yuan, X., Cardinale, S., Ribeiro, S., Lai, F., Ogawa, H., Gudmundsdottir, E., Gassmann, R., Morrison, C. G., Ruchaud, S., Earnshaw, W. C. (2008). Deconstructing Survivin: comprehensive genetic analysis of Survivin function by conditional knockout in a vertebrate cell line. JCB 183: 279-296 [Abstract] [Full Text]  
• Colombie, N., Cullen, C. F., Brittle, A. L., Jang, J. K., Earnshaw, W. C., Carmena, M., McKim, K., Ohkura, H. (2008). Dual roles of Incenp crucial to the assembly of the acentrosomal metaphase spindle in female meiosis. Development 135: 3239-3246 [Abstract] [Full Text]  
• Mita, A. C., Mita, M. M., Nawrocki, S. T., Giles, F. J. (2008). Survivin: Key Regulator of Mitosis and Apoptosis and Novel Target for Cancer Therapeutics. Clin. Cancer Res. 14: 5000-5005 [Abstract] [Full Text]  
• Fu, D.-H., Jiang, W., Zheng, J.-T., Zhao, G.-Y., Li, Y., Yi, H., Li, Z.-R., Jiang, J.-D., Yang, K.-Q., Wang, Y., Si, S.-Y. (2008). Jadomycin B, an Aurora-B kinase inhibitor discovered through virtual screening. Molecular Cancer Therapeutics 7: 2386-2393 [Abstract] [Full Text]  
• Yamamoto, T. M., Lewellyn, A. L., Maller, J. L. (2008). Regulation of the Aurora B Chromosome Passenger Protein Complex during Oocyte Maturation in Xenopus laevis. Mol. Cell. Biol. 28: 4196-4203 [Abstract] [Full Text]  
• Brennan, D. J., Rexhepaj, E., O'Brien, S. L., McSherry, E., O'Connor, D. P., Fagan, A., Culhane, A. C., Higgins, D. G., Jirstrom, K., Millikan, R. C., Landberg, G., Duffy, M. J., Hewitt, S. M., Gallagher, W. M. (2008). Altered Cytoplasmic-to-Nuclear Ratio of Survivin Is a Prognostic Indicator in Breast Cancer. Clin. Cancer Res. 14: 2681-2689 [Abstract] [Full Text]  
• Schmit, T. L, Ahmad, N. (2008). Regulation of Mitosis via Mitotic Kinases: New Opportunities for Cancer Management. aacredbook 2008: 133-150 [Abstract] [Full Text]  
• Gao, S., Giansanti, M. G., Buttrick, G. J., Ramasubramanyan, S., Auton, A., Gatti, M., Wakefield, J. G. (2008). Australin: a chromosomal passenger protein required specifically for Drosophila melanogaster male meiosis. JCB 180: 521-535 [Abstract] [Full Text]  
• Trinkle-Mulcahy, L., Lamond, A. I. (2007). Toward a High-Resolution View of Nuclear Dynamics. Science 318: 1402-1407 [Abstract] [Full Text]  
• Bourhis, E., Hymowitz, S. G., Cochran, A. G. (2007). The Mitotic Regulator Survivin Binds as a Monomer to Its Functional Interactor Borealin. J. Biol. Chem. 282: 35018-35023 [Abstract] [Full Text]  
• Vader, G., Cruijsen, C. W.A., van Harn, T., Vromans, M. J.M., Medema, R. H., Lens, S. M.A. (2007). The Chromosomal Passenger Complex Controls Spindle Checkpoint Function Independent from Its Role in Correcting Microtubule Kinetochore Interactions. Mol. Biol. Cell 18: 4553-4564 [Abstract] [Full Text]  
• Thomas, S., Kaplan, K. B. (2007). A Bir1p Sli15p Kinetochore Passenger Complex Regulates Septin Organization during Anaphase. Mol. Biol. Cell 18: 3820-3834 [Abstract] [Full Text]  
• Chen, Q., Lakshmikanth, G. S., Spudich, J. A., De Lozanne, A. (2007). The Localization of Inner Centromeric Protein (INCENP) at the Cleavage Furrow Is Dependent on Kif12 and Involves Interactions of the N Terminus of INCENP with the Actin Cytoskeleton. Mol. Biol. Cell 18: 3366-3374 [Abstract] [Full Text]  
• Wong, L. H., Brettingham-Moore, K. H., Chan, L., Quach, J. M., Anderson, M. A., Northrop, E. L., Hannan, R., Saffery, R., Shaw, M. L., Williams, E., Choo, K.H. A. (2007). Centromere RNA is a key component for the assembly of nucleoproteins at the nucleolus and centromere. Genome Res 17: 1146-1160 [Abstract] [Full Text]  
• Leung, C. G., Xu, Y., Mularski, B., Liu, H., Gurbuxani, S., Crispino, J. D. (2007). Requirements for survivin in terminal differentiation of erythroid cells and maintenance of hematopoietic stem and progenitor cells. JEM 204: 1603-1611 [Abstract] [Full Text]  
• Schmit, T. L., Ahmad, N. (2007). Regulation of mitosis via mitotic kinases: new opportunities for cancer management. Molecular Cancer Therapeutics 6: 1920-1931 [Abstract] [Full Text]  
• Pennati, M., Folini, M., Zaffaroni, N. (2007). Targeting survivin in cancer therapy: fulfilled promises and open questions. Carcinogenesis 28: 1133-1139 [Abstract] [Full Text]  
• Hayama, S., Daigo, Y., Yamabuki, T., Hirata, D., Kato, T., Miyamoto, M., Ito, T., Tsuchiya, E., Kondo, S., Nakamura, Y. (2007). Phosphorylation and Activation of Cell Division Cycle Associated 8 by Aurora Kinase B Plays a Significant Role in Human Lung Carcinogenesis. Cancer Res. 67: 4113-4122 [Abstract] [Full Text]  
• Perez de Castro, I., de Carcer, G., Malumbres, M. (2007). A census of mitotic cancer genes: new insights into tumor cell biology and cancer therapy. Carcinogenesis 28: 899-912 [Abstract] [Full Text]  
• Vanoosthuyse, V., Prykhozhij, S., Hardwick, K. G. (2007). Shugoshin 2 Regulates Localization of the Chromosomal Passenger Proteins in Fission Yeast Mitosis. Mol. Biol. Cell 18: 1657-1669 [Abstract] [Full Text]  
• Janicke, M. A., Lasko, L., Oldenbourg, R., LaFountain, J. R. Jr (2007). Chromosome Malorientations after Meiosis II Arrest Cause Nondisjunction. Mol. Biol. Cell 18: 1645-1656 [Abstract] [Full Text]  
• Takemoto, A., Murayama, A., Katano, M., Urano, T., Furukawa, K., Yokoyama, S., Yanagisawa, J., Hanaoka, F., Kimura, K. (2007). Analysis of the role of Aurora B on the chromosomal targeting of condensin I. Nucleic Acids Res 35: 2403-2412 [Abstract] [Full Text]  
• Faragher, A. J., Sun, X.-M., Butterworth, M., Harper, N., Mulheran, M., Ruchaud, S., Earnshaw, W. C., Cohen, G. M. (2007). Death Receptor-induced Apoptosis Reveals a Novel Interplay between the Chromosomal Passenger Complex and CENP-C during Interphase. Mol. Biol. Cell 18: 1337-1347 [Abstract] [Full Text]  
• Boyarchuk, Y., Salic, A., Dasso, M., Arnaoutov, A. (2007). Bub1 is essential for assembly of the functional inner centromere. JCB 176: 919-928 [Abstract] [Full Text]  
• Sakita-Suto, S., Kanda, A., Suzuki, F., Sato, S., Takata, T., Tatsuka, M. (2007). Aurora-B Regulates RNA Methyltransferase NSUN2. Mol. Biol. Cell 18: 1107-1117 [Abstract] [Full Text]  
• Krieg, A. J., Hammond, E. M., Giaccia, A. J. (2006). Functional Analysis of p53 Binding under Differential Stresses.. Mol. Cell. Biol. 26: 7030-7045 [Abstract] [Full Text]  
• Li, Z., Wang, C. C. (2006). Changing Roles of Aurora-B Kinase in Two Life Cycle Stages of Trypanosoma brucei.. Eukaryot Cell 5: 1026-1035 [Abstract] [Full Text]  
• Soncini, C., Carpinelli, P., Gianellini, L., Fancelli, D., Vianello, P., Rusconi, L., Storici, P., Zugnoni, P., Pesenti, E., Croci, V., Ceruti, R., Giorgini, M. L., Cappella, P., Ballinari, D., Sola, F., Varasi, M., Bravo, R., Moll, J. (2006). PHA-680632, a Novel Aurora Kinase Inhibitor with Potent Antitumoral Activity.. Clin. Cancer Res. 12: 4080-4089 [Abstract] [Full Text]  
• Vader, G., Medema, R. H., Lens, S. M.A. (2006). The chromosomal passenger complex: guiding Aurora-B through mitosis. JCB 173: 833-837 [Abstract] [Full Text]  
• Klein, U. R., Nigg, E. A., Gruneberg, U. (2006). Centromere Targeting of the Chromosomal Passenger Complex Requires a Ternary Subcomplex of Borealin, Survivin, and the N-Terminal Domain of INCENP. Mol. Biol. Cell 17: 2547-2558 [Abstract] [Full Text]  
• Burgess, A., Wigan, M., Giles, N., DePinto, W., Gillespie, P., Stevens, F., Gabrielli, B. (2006). Inhibition of S/G2 Phase CDK4 Reduces Mitotic Fidelity. J. Biol. Chem. 281: 9987-9995 [Abstract] [Full Text]  
• Lens, S. M.A., Rodriguez, J. A., Vader, G., Span, S. W., Giaccone, G., Medema, R. H. (2006). Uncoupling the Central Spindle-associated Function of the Chromosomal Passenger Complex from Its Role at Centromeres. Mol. Biol. Cell 17: 1897-1909 [Abstract] [Full Text]  
• Chang, C.-J., Goulding, S., Adams, R. R., Earnshaw, W. C., Carmena, M. (2006). Drosophila Incenp is required for cytokinesis and asymmetric cell division during development of the nervous system. J. Cell Sci. 119: 1144-1153 [Abstract] [Full Text]  
• Rosa, J., Canovas, P., Islam, A., Altieri, D. C., Doxsey, S. J. (2006). Survivin Modulates Microtubule Dynamics and Nucleation throughout the Cell Cycle. Mol. Biol. Cell 17: 1483-1493 [Abstract] [Full Text]  
• Trinkle-Mulcahy, L., Andersen, J., Lam, Y. W., Moorhead, G., Mann, M., Lamond, A. I. (2006). Repo-Man recruits PP1{gamma} to chromatin and is essential for cell viability. JCB 172: 679-692 [Abstract] [Full Text]  
• Chen, Q., Li, H., De Lozanne, A. (2006). Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis. Mol. Biol. Cell 17: 779-788 [Abstract] [Full Text]  
• Leslie, M. (2006). Passenger proteins check in. JCB 172: 329-329 [Full Text]  
• Noton, E. A., Colnaghi, R., Tate, S., Starck, C., Carvalho, A., Ferrigno, P. K., Wheatley, S. P. (2006). Molecular Analysis of Survivin Isoforms: EVIDENCE THAT ALTERNATIVELY SPLICED VARIANTS DO NOT PLAY A ROLE IN MITOSIS. J. Biol. Chem. 281: 1286-1295 [Abstract] [Full Text]  
• Nakashima, H., Nakano, M., Ohnishi, R., Hiraoka, Y., Kaneda, Y., Sugino, A., Masumoto, H. (2005). Assembly of additional heterochromatin distinct from centromere-kinetochore chromatin is required for de novo formation of human artificial chromosome. J. Cell Sci. 118: 5885-5898 [Abstract] [Full Text]  
• Gillis, A. N., Thomas, S., Hansen, S. D., Kaplan, K. B. (2005). A novel role for the CBF3 kinetochore-scaffold complex in regulating septin dynamics and cytokinesis. JCB 171: 773-784 [Abstract] [Full Text]  
• Vong, Q. P., Cao, K., Li, H. Y., Iglesias, P. A., Zheng, Y. (2005). Chromosome Alignment and Segregation Regulated by Ubiquitination of Survivin. Science 310: 1499-1504 [Abstract] [Full Text]  
• Hanson, K. K., Kelley, A. C., Bienz, M. (2005). Loss of Drosophila borealin causes polyploidy, delayed apoptosis and abnormal tissue development. Development 132: 4777-4787 [Abstract] [Full Text]  
• Huang, H.-K., Bailis, J. M., Leverson, J. D., Gomez, E. B., Forsburg, S. L., Hunter, T. (2005). Suppressors of Bir1p (Survivin) Identify Roles for the Chromosomal Passenger Protein Pic1p (INCENP) and the Replication Initiation Factor Psf2p in Chromosome Segregation. Mol. Cell. Biol. 25: 9000-9015 [Abstract] [Full Text]  
• Jang, J. K., Rahman, T., McKim, K. S. (2005). The Kinesinlike Protein Subito Contributes to Central Spindle Assembly and Organization of the Meiotic Spindle in Drosophila Oocytes. Mol. Biol. Cell 16: 4684-4694 [Abstract] [Full Text]  
• Saffin, J.-M., Venoux, M., Prigent, C., Espeut, J., Poulat, F., Giorgi, D., Abrieu, A., Rouquier, S. (2005). ASAP, a human microtubule-associated protein required for bipolar spindle assembly and cytokinesis. Proc. Natl. Acad. Sci. USA 102: 11302-11307 [Abstract] [Full Text]  
• Nguyen, H. G., Chinnappan, D., Urano, T., Ravid, K. (2005). Mechanism of Aurora-B Degradation and Its Dependency on Intact KEN and A-Boxes: Identification of an Aneuploidy-Promoting Property. Mol. Cell. Biol. 25: 4977-4992 [Abstract] [Full Text]  
• Yan, X., Cao, L., Li, Q., Wu, Y., Zhang, H., Saiyin, H., Liu, X., Zhang, X., Shi, Q., Yu, L. (2005). Aurora C is directly associated with Survivin and required for cytokinesis. GENES CELLS 10: 617-626 [Abstract] [Full Text]  
• Regnier, V., Vagnarelli, P., Fukagawa, T., Zerjal, T., Burns, E., Trouche, D., Earnshaw, W., Brown, W. (2005). CENP-A Is Required for Accurate Chromosome Segregation and Sustained Kinetochore Association of BubR1. Mol. Cell. Biol. 25: 3967-3981 [Abstract] [Full Text]  
• Uchiyama, S., Kobayashi, S., Takata, H., Ishihara, T., Hori, N., Higashi, T., Hayashihara, K., Sone, T., Higo, D., Nirasawa, T., Takao, T., Matsunaga, S., Fukui, K. (2005). Proteome Analysis of Human Metaphase Chromosomes. J. Biol. Chem. 280: 16994-17004 [Abstract] [Full Text]  
• Yanagida, M. (2005). Basic mechanism of eukaryotic chromosome segregation. Phil Trans R Soc B 360: 609-621 [Abstract] [Full Text]  
• Glotzer, M. (2005). The Molecular Requirements for Cytokinesis. Science 307: 1735-1739 [Abstract] [Full Text]  
• Mollinari, C., Kleman, J.-P., Saoudi, Y., Jablonski, S. A., Perard, J., Yen, T. J., Margolis, R. L. (2005). Ablation of PRC1 by Small Interfering RNA Demonstrates that Cytokinetic Abscission Requires a Central Spindle Bundle in Mammalian Cells, whereas Completion of Furrowing Does Not. Mol. Biol. Cell 16: 1043-1055 [Abstract] [Full Text]  
• Samuel, T., Okada, K., Hyer, M., Welsh, K., Zapata, J. M., Reed, J. C. (2005). cIAP1 Localizes to the Nuclear Compartment and Modulates the Cell Cycle. Cancer Res. 65: 210-218 [Abstract] [Full Text]  
• Scrittori, L., Skoufias, D. A., Hans, F., Gerson, V., Sassone-Corsi, P., Dimitrov, S., Margolis, R. L. (2005). A Small C-Terminal Sequence of Aurora B Is Responsible for Localization and Function. Mol. Biol. Cell 16: 292-305 [Abstract] [Full Text]  
• Li, X., Sakashita, G., Matsuzaki, H., Sugimoto, K., Kimura, K., Hanaoka, F., Taniguchi, H., Furukawa, K., Urano, T. (2004). Direct Association with Inner Centromere Protein (INCENP) Activates the Novel Chromosomal Passenger Protein, Aurora-C. J. Biol. Chem. 279: 47201-47211 [Abstract] [Full Text]  
• Maiato, H., DeLuca, J., Salmon, E. D., Earnshaw, W. C. (2004). The dynamic kinetochore-microtubule interface. J. Cell Sci. 117: 5461-5477 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents