Mouse centric and pericentric satellite repeats form distinct functional heterochromatin

doi:10.1083/jcb.200403109

Published online 9 August 2004. doi:10.1083/jcb.200403109
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 166, Number 4, 493-505

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Article

Mouse centric and pericentric satellite repeats form distinct functional heterochromatin

Mounia Guenatri, Delphine Bailly, Christèle Maison, and Geneviève Almouzni

Institut Curie/Research section, UMR218 du Centre National pour la Recherche Scientifique, 75248 Paris Cedex 05, France

Address correspondence to G. Almouzni, Institut Curie/Research section, UMR218 du Centre National pour la Recherche Scientifique, 26 rue d'Ulm, 75248 Paris Cedex 05, France. Tel.: 33 1 42 34 67 01. Fax: 33 1 46 33 30 16. email: almouzni{at}curie.fr

Heterochromatin is thought to play a critical role for centromericfunction. However, the respective contributions of the distinctrepetitive sequences found in these regions, such as minor andmajor satellites in the mouse, have remained largely unsolved.We show that these centric and pericentric repeats on the chromosomeshave distinct heterochromatic characteristics in the nucleus.Major satellites from different chromosomes form clusters associatedwith heterochromatin protein 1 ${alpha}$ , whereas minor satellites areindividual entities associated with centromeric proteins. Bothregions contain methylated histone H3 (Me-K9 H3) but show differentmicrococcal nuclease sensitivities. A dinucleosome repeatingunit is found specifically associated with major satellites.These domains replicate asynchronously, and chromatid cohesionis sustained for a longer time in major satellites comparedwith minor satellites. Such prolonged cohesion in major satellitesis lost in the absence of Suv39h histone methyltransferases.Thus, we define functionally independent centromeric subdomains,which spatio-temporal isolation is proposed to be importantfor centromeric cohesion and dissociation during chromosomesegregation.

Key Words: centromere; cohesion; replication; nuclear organization; cluster

Abbreviations used in this paper: ACA, anticentromere antibody;BiodU, Biotin-16-deoxyuridine; CENP, centromeric protein; di-Me-K9H3, histone H3 di-methylated at lysine 9; dn, double null; HP1,heterochromatin protein 1; Me-K9 H3, histone H3 methylated atlysine 9; Mnase, micrococcal nuclease; mono-Me-K9 H3, histoneH3 mono-methylated at lysine 9; NChIP, native chromatin immunoprecipitation;tri-Me-K9 H3, histone H3 tri-methylated at lysine 9.

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