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Published 30 August 2004. doi:10.1083/jcb.200406148
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 166, Number 5, 709-715
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Article

 Bend propagation drives central pair rotation in Chlamydomonas reinhardtii flagella

David R. Mitchell and Masako Nakatsugawa

Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210

Address correspondence to D.R. Mitchell, Dept. of Cell and Developmental Biology, State University of New York Upstate Medical University, 750 E. Adams St., Syracuse, NY 13210. Tel.: (315) 464-8575. Fax: (315) 464-8535. email: mitcheld{at}upstate.edu

Regulation of motile 9+2 cilia and flagella depends on interactions between radial spokes and a central pair apparatus. Although the central pair rotates during bend propagation in flagella of many organisms and rotation correlates with a twisted central pair structure, propulsive forces for central pair rotation and twist are unknown. Here we compared central pair conformation in straight, quiescent flagella to that in actively beating flagella using wild-type Chlamydomonas reinhardtii and mutants that lack radial spoke heads. Twists occur in quiescent flagella in both the presence and absence of spoke heads, indicating that spoke–central pair interactions are not needed to generate torque for twisting. Central pair orientation in propagating bends was also similar in wild type and spoke head mutant strains, thus orientation is a passive response to bend formation. These results indicate that bend propagation drives central pair rotation and suggest that dynein regulation by central pair–radial spoke interactions involves passive central pair reorientation to changes in bend plane.

Key Words: cilia; radial spoke; dynein; motility; microtubule

Abbreviation used in this paper: CP, central pair apparatus.


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