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Published 27 September 2004. doi:10.1083/jcb.200406060
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 166, Number 7, 1041-1054
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Article

Presenilin 1 mediates the turnover of telencephalin in hippocampal neurons via an autophagic degradative pathway



Cary Esselens1, Viola Oorschot3, Veerle Baert1, Tim Raemaekers1, Kurt Spittaels4, Lutgarde Serneels2, Hui Zheng6, Paul Saftig5, Bart De Strooper2, Judith Klumperman3, and Wim Annaert1

1 Membrane Trafficking Laboratory, Center for Human Genetics/VIB04, KULeuven, 3000 Leuven, Belgium
2 Neuronal Cell Biology and Gene Transfer Laboratory, Center for Human Genetics/VIB04, KULeuven, 3000 Leuven, Belgium
3 Department of Cell Biology, University Medical Center Utrecht, 3584CX Utrecht, Netherlands
4 Galapagos Genomics, B-2800 Mechelen, Belgium
5 Institute of Biochemistry, University of Kiel, D-24118 Kiel, Germany
6 Division of Neuroscience, Baylor College of Medicine, Houston, TX 77030

Address correspondence to Wim Annaert, Membrane Trafficking Laboratory, CME-VIB04, Gasthuisberg-KULeuven, 3000 Leuven, Belgium. Tel.: (32) 16-346371. Fax: (32) 16-347181. email: Willem.Annaert{at}med.kuleuven.ac.be

Presenilin 1 (PS1) interacts with telencephalin (TLN) and the amyloid precursor protein via their transmembrane domain (Annaert, W.G., C. Esselens, V. Baert, C. Boeve, G. Snellings, P. Cupers, K. Craessaerts, and B. De Strooper. 2001. Neuron. 32:579–589). Here, we demonstrate that TLN is not a substrate for {gamma}-secretase cleavage, but displays a prolonged half-life in PS1–/– hippocampal neurons. TLN accumulates in intracellular structures bearing characteristics of autophagic vacuoles including the presence of Apg12p and LC3. Importantly, the TLN accumulations are suppressed by adenoviral expression of wild-type, FAD-linked and D257A mutant PS1, indicating that this phenotype is independent from {gamma}-secretase activity. Cathepsin D deficiency also results in the localization of TLN to autophagic vacuoles. TLN mediates the uptake of microbeads concomitant with actin and PIP2 recruitment, indicating a phagocytic origin of TLN accumulations. Absence of endosomal/lysosomal proteins suggests that the TLN-positive vacuoles fail to fuse with endosomes/lysosomes, preventing their acidification and further degradation. Collectively, PS1 deficiency affects in a {gamma}-secretase–independent fashion the turnover of TLN through autophagic vacuoles, most likely by an impaired capability to fuse with lysosomes.

Key Words: autophagic vacuole; hippocampal neuron; phagocytosis; presenilin 1; telencephalin

Abbreviations used in this paper: APP, amyloid precursor protein; CatD, cathepsin D; CTF, COOH-terminal fragment; EndoH, endoglycosidase H; MDC, monodansylcadaverine; MOI, multiplicity of infection; PIP2, phosphatidylinositol 4,5 bisphosphate; PS, presenilin; SFV, Semliki Forest virus; TLN, telencephalin.


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