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Published 27 September 2004. doi:10.1083/jcb.200405159
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 166, Number 7, 1093-1102
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Article

Conversion of ES cells to columnar epithelia by hensin and to squamous epithelia by laminin



Jiro Takito and Qais Al-Awqati

Department of Medicine and Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032

Address correspondence to Qais Al-Awqati, Dept. of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th St., New York, NY 10032. Tel.: (212) 305-3512. Fax: (212) 305-3475. email: qa1{at}columbia.edu

Single-layered epithelia are the first differentiated cell types to develop in the embryo, with columnar and squamous types appearing immediately after blastocyst implantation. Here, we show that mouse embryonic stem cells seeded on hensin or laminin, but not fibronectin or collagen type IV, formed hemispheric epithelial structures whose outermost layer terminally differentiated to an epithelium that resembled the visceral endoderm. Hensin induced columnar epithelia, whereas laminin formed squamous epithelia. At the egg cylinder stage, the distal visceral endoderm is columnar, and these cells begin to migrate anteriorly to create the anterior visceral endoderm, which assumes a squamous shape. Hensin expression coincided with the dynamic appearance and disappearance of columnar cells at the egg cylinder stage of the embryo. These expression patterns, and the fact that hensin null embryos (and those already reported for laminin) die at the onset of egg cylinder formation, support the view that hensin and laminin are required for terminal differentiation of columnar and squamous epithelial phenotypes during early embryogenesis.

Key Words: epithelium; extracellular matrix proteins; cell size; embryonic development; stem cell

Abbreviations used in this paper: AVE, anterior visceral endoderm; E, embryonic day; ES, embryonic stem; ICM, inner cell mass; LIF, leukemia inhibitory factor.


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