The mechanism of spindle assembly: functions of Ran and its target TPX2

doi:10.1083/jcb.200312112

Published 27 September 2004. doi:10.1083/jcb.200312112
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 166, Number 7, 949-955

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The mechanism of spindle assembly

: functions of Ran and its target TPX2

Oliver J. Gruss¹ and Isabelle Vernos²

¹ Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg 69120, Germany
² Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Heidelberg 69117, Germany

Address correspondence to Isabelle Vernos, Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg 69117, Germany. Tel.: 49-6221-387-285. Fax: 49-6221-387-512. email: vernos{at}EMBL.de; or o.gruss{at}zmbh.uni-heidelberg.de

Abstract

Recent work has provided new insights into the mechanism ofspindle assembly. Growing evidence supports a model in whichthe small GTPase Ran plays a central role in this process. Here,we examine the evidence for the existence of a RanGTP gradientaround mitotic chromosomes and some controversial data on therole that chromosomes play in spindle assembly. We review thecurrent knowledge on the Ran downstream targets for spindleassembly and we focus on the multiple roles of TPX2, one ofthe targets of RanGTP during cell division.

Abbreviations used in this paper: KLP, kinesin-like protein;MAP, microtubule associated protein; NuMA, nuclear protein ofthe mitotic apparatus; RanGTP, GTPase Ran in its GTP-bound form;TPX2, targeting protein for Xklp2.

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