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Published online 1 November 2004. doi:10.1083/jcb.200405024
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 167, Number 3, 545-554
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Article

Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility



Lauren R. Strachan and Maureen L. Condic

Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132

Correspondence to Maureen Condic: maureen.condic{at}hsc.utah.edu

Cell migration is essential for proper development of numerous structures derived from embryonic neural crest cells (NCCs). Although the migratory pathways of NCCs have been determined, the molecular mechanisms regulating NCC motility remain unclear. NCC migration is integrin dependent, and recent work has shown that surface expression levels of particular integrin {alpha} subunits are important determinants of NCC motility in vitro. Here, we provide evidence that rapid cranial NCC motility on laminin requires integrin recycling. NCCs showed both ligand- and receptor-specific integrin regulation in vitro. On laminin, NCCs accumulated internalized laminin but not fibronectin receptors over 20 min, whereas on fibronectin neither type of receptor accumulated internally beyond 2 min. Internalized laminin receptors colocalized with receptor recycling vesicles and were subsequently recycled back to the cell surface. Blocking receptor recycling with bafilomycin A inhibited NCC motility on laminin, indicating that substratum-dependent integrin recycling is essential for rapid cranial neural crest migration.

Abbreviations used in this paper: BafA, bafilomycin A; FN1, fibronectin applied at 1 µg/ml (low fibronectin); FN20, fibronectin applied at 20 µg/ml (high fibronectin; LM1, laminin applied at 1 µg/ml (low laminin); LM20, laminin applied at 20 µg/ml (high laminin); MesNa, sodium 2-mercaptoethanesulfonate; NCC, neural crest cell.


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