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Published 6 December 2004. doi:10.1083/jcb.200407126
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 167, Number 5, 813-818
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The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles



David T. Miyamoto1, Zachary E. Perlman1, Kendra S. Burbank1,2, Aaron C. Groen1, and Timothy J. Mitchison1

1 Department of Systems Biology, Harvard Medical School, Boston, MA 02115
2 Department of Physics, Harvard University, Cambridge, MA 02138

Correspondence to David T. Miyamoto: miyamoto@post.harvard.edu


Abstract

Although mitotic and meiotic spindles maintain a steady-state length during metaphase, their antiparallel microtubules slide toward spindle poles at a constant rate. This "poleward flux" of microtubules occurs in many organisms and may provide part of the force for chromosome segregation. We use quantitative image analysis to examine the role of the kinesin Eg5 in poleward flux in metaphase Xenopus laevis egg extract spindles. Pharmacological inhibition of Eg5 results in a dose–responsive slowing of flux, and biochemical depletion of Eg5 significantly decreases the flux rate. Our results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles.

Abbreviation used in this paper: FSM, fluorescent speckle microscopy.


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