JCB logo
amgmicro.com
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 20 December 2004. doi:10.1083/jcb.200408008
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 167, Number 6, 1005-1010
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1613K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, Y. J.
Right arrow Articles by Yin, H. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, Y. J.
Right arrow Articles by Yin, H. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Report

Critical role of PIP5KI{gamma}87 in InsP3-mediated Ca2+ signaling



Ying Jie Wang1, Wen Hong Li2, Jing Wang1, Ke Xu2, Ping Dong1, Xiang Luo1, and Helen L. Yin1

1 Department of Physiology and 2 Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390

Correspondence to Helen L. Yin: Helen.Yin{at}UTSouthwestern.edu


Abstract

Phosphatidylinositol 4,5-bisphosphate (PIP2) is the obligatory precursor of inositol 1,4,5-trisphosphate (InsP3 or IP3) and is therefore critical to intracellular Ca2+ signaling. Using RNA interference (RNAi), we identified the short splice variant of type I phosphatidylinositol 4-phosphate 5-kinase {gamma} (PIP5KI{gamma}87) as the major contributor of the PIP2 pool that supports G protein–coupled receptor (GPCR)-mediated IP3 generation. PIP5KI{gamma}87 RNAi decreases the histamine-induced IP3 response and Ca2+ flux by 70%. Strikingly, RNAi of other PIP5KI isoforms has minimal effect, even though some of these isoforms account for a larger percent of total PIP2 mass and have previously been implicated in receptor mediated endocytosis or focal adhesion formation. Therefore, PIP5KI{gamma}87's PIP2 pool that supports GPCR-mediated Ca2+ signaling is functionally compartmentalized from those generated by the other PIP5KIs.

Abbreviations used in this paper: [Ca2+]i, intracellular Ca2+ concentration; GPCR, G protein–coupled receptor; IP3, inositol 1,4,5-trisphosphate; PIP5KI{gamma}, type I phosphatidylinositol 4-phosphate 5-kinase {gamma}; PIP2, phosphatidylinositol 4,5-bisphosphate; PM, plasma membrane; RNAi, RNA interference; siRNA, small interfering RNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents