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Published 18 January 2005. doi:10.1083/jcb.200408087
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 168, Number 2, 209-219
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Article

Spindle-independent condensation-mediated segregation of yeast ribosomal DNA in late anaphase



Félix Machín, Jordi Torres-Rosell, Adam Jarmuz, and Luis Aragón

Cell Cycle Group, Clinical Sciences Centre, Medical Research Council, Imperial College London, London W12 0NN, England, UK

Correspondence to Luis Aragón: luis.aragon{at}csc.mrc.ac.uk

Mitotic cell division involves the equal segregation of all chromosomes during anaphase. The presence of ribosomal DNA (rDNA) repeats on the right arm of chromosome XII makes it the longest in the budding yeast genome. Previously, we identified a stage during yeast anaphase when rDNA is stretched across the mother and daughter cells. Here, we show that resolution of sister rDNAs is achieved by unzipping of the locus from its centromere-proximal to centromere-distal regions. We then demonstrate that during this stretched stage sister rDNA arrays are neither compacted nor segregated despite being largely resolved from each other. Surprisingly, we find that rDNA segregation after this period no longer requires spindles but instead involves Cdc14-dependent rDNA axial compaction. These results demonstrate that chromosome resolution is not simply a consequence of compacting chromosome arms and that overall rDNA compaction is necessary to mediate the segregation of the long arm of chromosome XII.

Abbreviations used in this paper: rDNA, ribosomal DNA; SPB, spindle pole body.


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