JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 7 February 2005. doi:10.1083/jcb.200405120
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 168, Number 4, 567-573
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2349K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stramer, B.
Right arrow Articles by Martin, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stramer, B.
Right arrow Articles by Martin, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Report

Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration



Brian Stramer1,2, Will Wood3, Michael J. Galko4,5, Michael J. Redd6, Antonio Jacinto3, Susan M. Parkhurst7, and Paul Martin1,2,6

1 Department of Physiology, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK
2 Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK
3 Centro Biologia Desenvolvimento, Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal
4 Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305
5 Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305
6 Department of Anatomy, University College London, London, WC1T 6BT, UK
7 Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109

Correspondence to Paul Martin: paul.martin{at}bristol.ac.uk


Abstract

Aa robust inflammatory response to tissue damage and infection is conserved across almost all animal phyla. Neutrophils and macrophages, or their equivalents, are drawn to the wound site where they engulf cell and matrix debris and release signals that direct components of the repair process. This orchestrated cell migration is clinically important, and yet, to date, leukocyte chemotaxis has largely been studied in vitro. Here, we describe a genetically tractable in vivo wound model of inflammation in the Drosophila melanogaster embryo that is amenable to cinemicroscopy. For the first time, we are able to examine the roles of Rho-family small GTPases during inflammation in vivo and show that Rac-mediated lamellae are essential for hemocyte motility and Rho signaling is necessary for cells to retract from sites of matrix– and cell–cell contacts. Cdc42 is necessary for maintaining cellular polarity and yet, despite in vitro evidence, is dispensable for sensing and crawling toward wound cues.

B. Stramer and W. Wood contributed equally to this paper.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents