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Published online 21 March 2005. doi:10.1083/jcb.200409138
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 168, Number 7, 1039-1051
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Article

Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE



Akira Honda1, Omayma S. Al-Awar1, Jesse C. Hay2, and Julie G. Donaldson1

1 Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health (NIH), Bethesda, MD 20892
2 Division of Biological Sciences, University of Montana, Missoula, MT 59812

Correspondence to Julie G. Donaldson: jdonalds{at}helix.nih.gov

Arf and Rab family GTPases regulate membrane traffic in cells, yet little is known about how they are targeted to distinct organelles. To identify sequences in Arf-1 necessary for Golgi targeting, we examined the localization of chimeras between Arf-1 and Arf-6. Here, we identify a 16–amino acid sequence in Arf-1 that specifies Golgi targeting and contains a motif (MXXE) that is important for Arf-1 binding to membrin, an ER-Golgi SNARE protein. The MXXE motif is conserved in all Arfs known to localize to the Golgi and enables Arf-1 to localize to the early Golgi. Arf-1 lacking these 16 aa can still localize to the late Golgi where it displays a more rapid Golgi-cytosol cycle than wild-type Arf-1. These studies suggest that membrin recruits Arf-1 to the early Golgi and reveal distinct kinetic cycles for Arf-1 at early and late Golgi determined by different sets of Arf regulators and effectors.

Abbreviations used in this paper: BFA, Brefeldin A; BIG, BFA-inhibited GEF; CD, circular dichroism; COPI, coat protein I; COPII, coat protein II; DSP, dithiobis(succinimidyl propionate); GalT, galactosyltransferase; GAP, GTPase-activating protein; GBF1, Golgi-specific BFA resistance factor 1; GEF, guanine nucleotide exchange factor; GGA, Golgi-localized, {gamma}-ear–containing, Arf-binding proteins.


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