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MCAK associates with the tips of polymerizing microtubules

¹ Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195
² Center for Cell Dynamics, Friday Harbor Labs, University of Washington, Friday Harbor, WA 98250
³ Laboratoire du Cytosquelette, INSERM U366, 38054 Grenoble, France

Correspondence to Linda Wordeman: worde{at}u.washington.edu

Abstract

MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-terminal tail of MCAK. Tip tracking is not essential for MCAK's MT-depolymerizing activity. We propose that tip tracking is a mechanism by which MCAK is preferentially localized to regions of the cell that modulate the plus ends of MTs.

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