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Published online 16 May 2005. doi:10.1083/jcb.200410081
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 169, Number 4, 635-646
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Article

Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells



Slobodan Beronja1, Patrick Laprise1, Ophelia Papoulas2,3, Milena Pellikka1, John Sisson2,3, and Ulrich Tepass1

1 Department of Zoology, University of Toronto, Toronto, Ontario M5S 3G5, Canada
2 The Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, Austin, TX 78712
3 The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712

Correspondence to Ulrich Tepass: utepass{at}zoo.utoronto.ca

Polarized exocytosis plays a major role in development and cell differentiation but the mechanisms that target exocytosis to specific membrane domains in animal cells are still poorly understood. We characterized Drosophila Sec6, a component of the exocyst complex that is believed to tether secretory vesicles to specific plasma membrane sites. sec6 mutations cause cell lethality and disrupt plasma membrane growth. In developing photoreceptor cells (PRCs), Sec6 but not Sec5 or Sec8 shows accumulation at adherens junctions. In late PRCs, Sec6, Sec5, and Sec8 colocalize at the rhabdomere, the light sensing subdomain of the apical membrane. PRCs with reduced Sec6 function accumulate secretory vesicles and fail to transport proteins to the rhabdomere, but show normal localization of proteins to the apical stalk membrane and the basolateral membrane. Furthermore, we show that Rab11 forms a complex with Sec5 and that Sec5 interacts with Sec6 suggesting that the exocyst is a Rab11 effector that facilitates protein transport to the apical rhabdomere in Drosophila PRCs.

Abbreviations used in this paper: AEL, after egg laying; RE, recycling endosome; Arm, Armadillo; Crb, Crumbs; Chp, Chaoptin; D{alpha}cat, D{alpha}-catenin; DEcad, DE-cadherin; MF, morphogenetic furrow; PD, pupal development; Rh1, Rhodopsin 1; PRC, photoreceptor cell; ZA, zonula adherens.


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