SNAREs can promote complete fusion and hemifusion as alternative outcomes

doi:10.1083/jcb.200501093

Quantitative Colocalization Analysis Software

Published 18 July 2005. doi:10.1083/jcb.200501093
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 170, Number 2, 249-260

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Article

SNAREs can promote complete fusion and hemifusion as alternative outcomes

Claudio G. Giraudo¹, Chuan Hu¹, Daoqi You³, Avram M. Slovic¹, Eugene V. Mosharov², David Sulzer², Thomas J. Melia¹, and James E. Rothman¹

¹ Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032
² Department of Neurology, Columbia University College of Physicians and Surgeons, New York, NY 10032
³ Memorial Sloan-Kettering Cancer Center, Long Island City, NY 11101

Correspondence to James E. Rothman: jr2269{at}columbia.edu

Using a cell fusion assay, we show here that in addition tocomplete fusion SNAREs also promote hemifusion as an alternativeoutcome. Approximately 65% of events resulted in full fusion,and the remaining 35% in hemifusion; of those, approximatelytwo thirds were permanent and approximately one third were reversible.We predict that this relatively close balance among outcomescould be tipped by binding of regulatory proteins to the SNAREs,allowing for dynamic physiological regulation between full fusionand reversible kiss-and-run–like events.

C.G. Giraudo and C. Hu contributed equally to this paper.

Abbreviations used in this paper: CMFDA, 5-chloromethylfluoresceindiacetate; GPI, glycosylphosphatidylinositol; PI-PLC, phosphatidylinositol-specificphospholipase C; TMD, transmembrane domain.

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