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Published online 8 August 2005. doi:10.1083/jcb.200502053
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 170, Number 4, 637-648
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Article

Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast



Vladimir Sirotkin1, Christopher C. Beltzner1, Jean-Baptiste Marchand4, and Thomas D. Pollard1,2,3

1 Departments of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520
2 Departments of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520
3 Departments of Cell Biology, Yale University, New Haven, CT 06520
4 Avidis, Biopole Clermont-Limagne, 63360 Saint Beauzire, France

Correspondence to Thomas D. Pollard: thomas.pollard{at}yale.edu

Yeast actin patches are dynamic structures that form at the sites of cell growth and are thought to play a role in endocytosis. We used biochemical analysis and live cell imaging to investigate actin patch assembly in fission yeast Schizosaccharomyces pombe. Patch assembly proceeds via two parallel pathways: one dependent on WASp Wsp1p and verprolin Vrp1p converges with another dependent on class 1 myosin Myo1p to activate the actin-related protein 2/3 (Arp2/3) complex. Wsp1p activates Arp2/3 complex via a conventional mechanism, resulting in branched filaments. Myo1p is a weaker Arp2/3 complex activator that makes unstable branches and is enhanced by verprolin. During patch assembly in vivo, Wsp1p and Vrp1p arrive first independent of Myo1p. Arp2/3 complex associates with nascent activator patches over 6–9 s while remaining stationary. After reaching a maximum concentration, Arp2/3 complex patches move centripetally as activator proteins dissociate. Genetic dependencies of patch formation suggest that patch formation involves cross talk between Myo1p and Wsp1p/Vrp1p pathways.

Abbreviations used in this paper: Arp2/3, actin-related protein 2/3; CA, central-acidic; mGFP, monomeric GFP; N-WASP, neuronal WASp; SH3, Src homology 3; TH, tail homology; WASp, Wiskott-Aldrich Syndrome protein; WH2, WASp homology 2.


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