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Published 15 August 2005. doi:10.1083/jcb.200412021
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 170, Number 4, 649-659
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Article

Intraflagellar transport (IFT) during assembly and disassembly of Chlamydomonas flagella



William Dentler

Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66049

Correspondence to William Dentler: wdent{at}ku.edu

Intraflagellar transport (IFT) of particles along flagellar microtubules is required for the assembly and maintenance of eukaryotic flagella and cilia. In Chlamydomonas, anterograde and retrograde particles viewed by light microscopy average 0.12-µm and 0.06-µm diameter, respectively. Examination of IFT particle structure in growing flagella by electron microscopy revealed similar size aggregates composed of small particles linked to each other and to the membrane and microtubules. To determine the relationship between the number of particles and flagellar length, the rate and frequency of IFT particle movement was measured in nongrowing, growing, and shortening flagella. In all flagella, anterograde and retrograde IFT averaged 1.9 µm/s and 2.7 µm/s, respectively, but retrograde IFT was significantly slower in flagella shorter than 4 µm. The number of flagellar IFT particles was not fixed, but depended on flagellar length. Pauses in IFT particle entry into flagella suggest the presence of a periodic "gate" that permits up to 4 particles/s to enter a flagellum.


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