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Published 26 September 2005. doi:10.1083/jcb.200504097
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 170, Number 7, 1039-1046
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Article

 Aurora A activates D-TACC–Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules

Teresa P. Barros1, Kazuhisa Kinoshita2, Anthony A. Hyman2, and Jordan W. Raff1

1 The Wellcome Trust/Cancer Research UK Gurdon Institute, Department of Genetics, Cambridge CB2 1QN, England, UK
2 Max Planck Institute of Molecular Cell Biology and Genetics, D-01307 Dresden, Germany

Correspondence to Jordan W. Raff: j.raff{at}gurdon.cam.ac.uk

Centrosomes are the dominant sites of microtubule (MT) assembly during mitosis in animal cells, but it is unclear how this is achieved. Transforming acidic coiled coil (TACC) proteins stabilize MTs during mitosis by recruiting Minispindles (Msps)/XMAP215 proteins to centrosomes. TACC proteins can be phosphorylated in vitro by Aurora A kinases, but the significance of this remains unclear. We show that Drosophila melanogaster TACC (D-TACC) is phosphorylated on Ser863 exclusively at centrosomes during mitosis in an Aurora A–dependent manner. In embryos expressing only a mutant form of D-TACC that cannot be phosphorylated on Ser863 (GFP-S863L), spindle MTs are partially destabilized, whereas astral MTs are dramatically destabilized. GFP-S863L is concentrated at centrosomes and recruits Msps there but cannot associate with the minus ends of MTs. We propose that the centrosomal phosphorylation of D-TACC on Ser863 allows D-TACC–Msps complexes to stabilize the minus ends of centrosome-associated MTs. This may explain why centrosomes are such dominant sites of MT assembly during mitosis.

Abbreviations used in this paper: D-TACC, Drosophila melanogaster TACC; Msps, minispindles; MT, microtubule; P–D-TACC, phospho–D-TACC; TACC, transforming acidic coiled coil; X-TACC, Xenopus laevis TACC.


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