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Published online 19 September 2005. doi:10.1083/jcb.200503023
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 170, Number 7, 1047-1055
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Article

 Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis

Kazuhisa Kinoshita1, Tim L. Noetzel1, Laurence Pelletier1, Karl Mechtler2, David N. Drechsel1, Anne Schwager1, Mike Lee3, Jordan W. Raff3, and Anthony A. Hyman1

1 Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany
2 Research Institute of Molecular Pathology, A-1030 Vienna, Austria
3 The Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge CB2 1QN, England, UK

Correspondence to Kazuhisa Kinoshita: kinoshita{at}mpi-cbg.de

Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3–XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.

Abbreviations used in this paper: D-TACC, Drosophila melanogaster TACC; FSG, fish skin gelatin; MCAK, mitotic centromere-associated kinesin; TACC, transforming acidic coiled coil; TOG, tumor overexpressed gene; WT, wild type.


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