Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis

doi:10.1083/jcb.200503023

Published online 19 September 2005. doi:10.1083/jcb.200503023
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 170, Number 7, 1047-1055

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Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis

Kazuhisa Kinoshita¹, Tim L. Noetzel¹, Laurence Pelletier¹, Karl Mechtler², David N. Drechsel¹, Anne Schwager¹, Mike Lee³, Jordan W. Raff³, and Anthony A. Hyman¹

¹ Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany
² Research Institute of Molecular Pathology, A-1030 Vienna, Austria
³ The Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge CB2 1QN, England, UK

Correspondence to Kazuhisa Kinoshita: kinoshita{at}mpi-cbg.de

Centrosomes act as sites of microtubule growth, but little isknown about how the number and stability of microtubules emanatingfrom a centrosome are controlled during the cell cycle. We studiedthe role of the TACC3–XMAP215 complex in this processby using purified proteins and Xenopus laevis egg extracts.We show that TACC3 forms a one-to-one complex with and enhancesthe microtubule-stabilizing activity of XMAP215 in vitro. TACC3enhances the number of microtubules emanating from mitotic centrosomes,and its targeting to centrosomes is regulated by Aurora A–dependentphosphorylation. We propose that Aurora A regulation of TACC3activity defines a centrosome-specific mechanism for regulationof microtubule polymerization in mitosis.

Abbreviations used in this paper: D-TACC, Drosophila melanogasterTACC; FSG, fish skin gelatin; MCAK, mitotic centromere-associatedkinesin; TACC, transforming acidic coiled coil; TOG, tumor overexpressedgene; WT, wild type.

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