Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 5410K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by de Rooij, J. Articles by Waterman-Storer, C. M. Search for Related Content PubMed PubMed Citation Articles by de Rooij, J. Articles by Waterman-Storer, C. M. Related Collections Related Article Social Bookmarking                            What's this?

Integrin-dependent actomyosin contraction regulates epithelial cell scattering

¹ Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
² Department of Microbiology, Cardiovascular Research Center, Mellon Prostate Cancer Institute, University of Virginia, Charlottesville, VA 22908
³ Department of Biomedical Engineering, Cardiovascular Research Center, Mellon Prostate Cancer Institute, University of Virginia, Charlottesville, VA 22908

Correspondence to Johan de Rooij: j.d.rooij{at}nki.nl

The scattering of Madin-Darby canine kidney cells in vitro mimics key aspects of epithelial–mesenchymal transitions during development, carcinoma cell invasion, and metastasis. Scattering is induced by hepatocyte growth factor (HGF) and is thought to involve disruption of cadherin-dependent cell–cell junctions. Scattering is enhanced on collagen and fibronectin, as compared with laminin1, suggesting possible cross talk between integrins and cell–cell junctions. We show that HGF does not trigger any detectable decrease in E-cadherin function, but increases integrin-mediated adhesion. Time-lapse imaging suggests that tension on cell–cell junctions may disrupt cell–cell adhesion. Varying the density and type of extracellular matrix proteins shows that scattering correlates with stronger integrin adhesion and increased phosphorylation of the myosin regulatory light chain. To directly test the role of integrin-dependent traction forces, substrate compliance was varied. Rigid substrates that produce high traction forces promoted scattering, in comparison to more compliant substrates. We conclude that integrin-dependent actomyosin traction force mediates the disruption of cell–cell adhesion during epithelial cell scattering.

J. de Rooij's present address is Dept. of Cell Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.

A. Kersten's present address is University of Texas at El Paso, College of Engineering, El Paso, TX 79968.

Abbreviations used in this paper: 2D, two-dimensional; CCD, charge-coupled device; Cn, collagen; EMT, epithelial–mesenchymal transition; Fn, fibronectin; HGF, hepatocyte growth factor; Ln, laminin; MLC, myosin II regulatory light chain; Vn, vitronectin; ZO-1, zona-occludens-1.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

Related Article

Breaking cell bonds

Nicole LeBrasseur
J. Cell Biol. 2005 171: 8. [Full Text] [PDF]

This article has been cited by other articles:

• Martinez-Rico, C., Pincet, F., Thiery, J.-P., Dufour, S. (2010). Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractility. J. Cell Sci. 123: 712-722 [Abstract] [Full Text]  
• Cai, Y., Rossier, O., Gauthier, N. C., Biais, N., Fardin, M.-A., Zhang, X., Miller, L. W., Ladoux, B., Cornish, V. W., Sheetz, M. P. (2010). Cytoskeletal coherence requires myosin-IIA contractility. J. Cell Sci. 123: 413-423 [Abstract] [Full Text]  
• Bradley, W. D., Koleske, A. J. (2009). Regulation of cell migration and morphogenesis by Abl-family kinases: emerging mechanisms and physiological contexts. J. Cell Sci. 122: 3441-3454 [Abstract] [Full Text]  
• Lapetina, S., Mader, C. C., Machida, K., Mayer, B. J., Koleske, A. J. (2009). Arg interacts with cortactin to promote adhesion-dependent cell edge protrusion. JCB 185: 503-519 [Abstract] [Full Text]  
• Engler, A. J., Humbert, P. O., Wehrle-Haller, B., Weaver, V. M. (2009). Multiscale Modeling of Form and Function. Science 324: 208-212 [Abstract] [Full Text]  
• Ryu, J. R., Echarri, A., Li, R., Pendergast, A. M. (2009). Regulation of Cell-Cell Adhesion by Abi/Diaphanous Complexes. Mol. Cell. Biol. 29: 1735-1748 [Abstract] [Full Text]  
• Busche, S., Descot, A., Julien, S., Genth, H., Posern, G. (2008). Epithelial cell-cell contacts regulate SRF-mediated transcription via Rac-actin-MAL signalling. J. Cell Sci. 121: 1025-1035 [Abstract] [Full Text]  
• de Graauw, M., Tijdens, I., Smeets, M. B., Hensbergen, P. J., Deelder, A. M., van de Water, B. (2008). Annexin A2 Phosphorylation Mediates Cell Scattering and Branching Morphogenesis via Cofilin Activation. Mol. Cell. Biol. 28: 1029-1040 [Abstract] [Full Text]  
• Schlunck, G., Han, H., Wecker, T., Kampik, D., Meyer-ter-Vehn, T., Grehn, F. (2008). Substrate Rigidity Modulates Cell Matrix Interactions and Protein Expression in Human Trabecular Meshwork Cells. IOVS 49: 262-269 [Abstract] [Full Text]  
• Zandy, N. L., Playford, M., Pendergast, A. M. (2007). Abl tyrosine kinases regulate cell cell adhesion through Rho GTPases. Proc. Natl. Acad. Sci. USA 104: 17686-17691 [Abstract] [Full Text]  
• Poujade, M., Grasland-Mongrain, E., Hertzog, A., Jouanneau, J., Chavrier, P., Ladoux, B., Buguin, A., Silberzan, P. (2007). From the Cover: Collective migration of an epithelial monolayer in response to a model wound. Proc. Natl. Acad. Sci. USA 104: 15988-15993 [Abstract] [Full Text]  
• Gupton, S. L., Eisenmann, K., Alberts, A. S., Waterman-Storer, C. M. (2007). mDia2 regulates actin and focal adhesion dynamics and organization in the lamella for efficient epithelial cell migration. J. Cell Sci. 120: 3475-3487 [Abstract] [Full Text]  
• Peacock, J. G., Miller, A. L., Bradley, W. D., Rodriguez, O. C., Webb, D. J., Koleske, A. J. (2007). The Abl-related Gene Tyrosine Kinase Acts through p190RhoGAP to Inhibit Actomyosin Contractility and Regulate Focal Adhesion Dynamics upon Adhesion to Fibronectin. Mol. Biol. Cell 18: 3860-3872 [Abstract] [Full Text]  
• Samarin, S. N., Ivanov, A. I., Flatau, G., Parkos, C. A., Nusrat, A. (2007). Rho/Rho-associated Kinase-II Signaling Mediates Disassembly of Epithelial Apical Junctions. Mol. Biol. Cell 18: 3429-3439 [Abstract] [Full Text]  
• Alderliesten, M., de Graauw, M., Oldenampsen, J., Qin, Y., Pont, C., van Buren, L., van de Water, B. (2007). Extracellular Signal-Regulated Kinase Activation during Renal Ischemia/Reperfusion Mediates Focal Adhesion Dissolution and Renal Injury. Am. J. Pathol. 171: 452-462 [Abstract] [Full Text]  
• Cohen, D., Tian, Y., Musch, A. (2007). Par1b Promotes Hepatic-type Lumen Polarity in Madin Darby Canine Kidney Cells via Myosin II- and E-Cadherin-dependent Signaling. Mol. Biol. Cell 18: 2203-2215 [Abstract] [Full Text]  
• Grande-Garcia, A., Echarri, A., de Rooij, J., Alderson, N. B., Waterman-Storer, C. M., Valdivielso, J. M., del Pozo, M. A. (2007). Caveolin-1 regulates cell polarization and directional migration through Src kinase and Rho GTPases. JCB 177: 683-694 [Abstract] [Full Text]  
• Saez, A., Ghibaudo, M., Buguin, A., Silberzan, P., Ladoux, B. (2007). Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates. Proc. Natl. Acad. Sci. USA 104: 8281-8286 [Abstract] [Full Text]  
• Fan, L., Sebe, A., Peterfi, Z., Masszi, A., Thirone, A. C.P., Rotstein, O. D., Nakano, H., McCulloch, C. A., Szaszi, K., Mucsi, I., Kapus, A. (2007). Cell Contact-dependent Regulation of Epithelial-Myofibroblast Transition via the Rho-Rho Kinase-Phospho-Myosin Pathway. Mol. Biol. Cell 18: 1083-1097 [Abstract] [Full Text]  
• Shtutman, M., Levina, E., Ohouo, P., Baig, M., Roninson, I. B. (2006). Cell Adhesion Molecule L1 Disrupts E-Cadherin-Containing Adherens Junctions and Increases Scattering and Motility of MCF7 Breast Carcinoma Cells. Cancer Res. 66: 11370-11380 [Abstract] [Full Text]  
• Mazzone, M., Comoglio, P. M. (2006). The Met pathway: master switch and drug target in cancer progression. FASEB J. 20: 1611-1621 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents