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Published 10 October 2005. doi:10.1083/jcb.200503139
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 171, Number 1, 47-59
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Article

 Microdomains bounded by endoplasmic reticulum segregate cell cycle calcium transients in syncytial Drosophila embryos

Huw Parry, Alex McDougall, and Michael Whitaker

Institute for Cell and Molecular Biosciences, University of Newcastle upon Tyne Medical School, Newcastle upon Tyne NE2 4HH, England, UK

Correspondence to Michael Whitaker: michael.whitaker{at}ncl.ac.uk

Cell cycle calcium signals are generated by the inositol trisphosphate (InsP3)–mediated release of calcium from internal stores (Ciapa, B., D. Pesando, M. Wilding, and M. Whitaker. 1994. Nature. 368:875–878; Groigno, L., and M. Whitaker. 1998. Cell. 92:193–204). The major internal calcium store is the endoplasmic reticulum (ER); thus, the spatial organization of the ER during mitosis may be important in shaping and defining calcium signals. In early Drosophila melanogaster embryos, ER surrounds the nucleus and mitotic spindle during mitosis, offering an opportunity to determine whether perinuclear localization of ER conditions calcium signaling during mitosis. We establish that the nuclear divisions in syncytial Drosophila embryos are accompanied by both cortical and nuclear localized calcium transients. Constructs that chelate InsP3 also prevent nuclear division. An analysis of nuclear calcium concentrations demonstrates that they are differentially regulated. These observations demonstrate that mitotic calcium signals in Drosophila embryos are confined to mitotic microdomains and offer an explanation for the apparent absence of detectable global calcium signals during mitosis in some cell types.

Abbreviations used in this paper: CaGr, calcium green dextran; [Cai], intracellular free calcium concentration; DiIC18, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; InsP3, inositol trisphosphate; NEB, nuclear envelope breakdown; TMR, tetramethylrhodamine.


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