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Published 24 October 2005. doi:10.1083/jcb.200506194
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 171, Number 2, 255-265
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Article

 Regulation of meiotic prophase arrest in mouse oocytes by GPR3, a constitutive activator of the Gs G protein

Leon Freudzon1, Rachael P. Norris1, Arthur R. Hand2, Shigeru Tanaka3, Yoshinaga Saeki3, Teresa L.Z. Jones4, Mark M. Rasenick5,6, Catherine H. Berlot7, Lisa M. Mehlmann1, and Laurinda A. Jaffe1

1 Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06032
2 Department of Pediatric Dentistry, University of Connecticut Health Center, Farmington, CT 06032
3 Department of Neurological Surgery, The Ohio State University, Columbus, OH 43210
4 National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
5 Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612
6 Department of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612
7 Weis Center for Research, Geisinger Clinic, Danville, PA 17822

Correspondence to Laurinda A. Jaffe: ljaffe{at}neuron.uchc.edu

The arrest of meiotic prophase in mouse oocytes within antral follicles requires the G protein Gs and an orphan member of the G protein–coupled receptor family, GPR3. To determine whether GPR3 activates Gs, the localization of G{alpha}s in follicle-enclosed oocytes from Gpr3+/+ and Gpr3–/– mice was compared by using immunofluorescence and G{alpha}sGFP. GPR3 decreased the ratio of G{alpha}s in the oocyte plasma membrane versus the cytoplasm and also decreased the amount of G{alpha}s in the oocyte. Both of these properties indicate that GPR3 activates Gs. The follicle cells around the oocyte are also necessary to keep the oocyte in prophase, suggesting that they might activate GPR3. However, GPR3-dependent Gs activity was similar in follicle-enclosed and follicle-free oocytes. Thus, the maintenance of prophase arrest depends on the constitutive activity of GPR3 in the oocyte, and the follicle cell signal acts by a means other than increasing GPR3 activity.

L. Freudzon and R.P. Norris contributed equally to this paper.

Abbreviations used in this paper: eCG, equine chorionic gonadotropin; PDE, phosphodiesterase.


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