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Published 7 November 2005. doi:10.1083/jcb.200506185
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 171, Number 3, 437-445
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Article

 Centrobin : a novel daughter centriole–associated protein that is required for centriole duplication



Chaozhong Zou1, Jun Li1, Yujie Bai1, William T. Gunning2, David E. Wazer3, Vimla Band1, and Qingshen Gao1

1 Department of Medicine, Division of Cancer Biology, Evanston Northwestern Healthcare Research Institute, Northwestern University Feinberg School of Medicine, Evanston, IL 60201
2 Department of Pathology, Biochemistry, and Cancer Biology, Medical College of Ohio, Toledo, OH 43614
3 Department of Radiation Oncology, New England Medical Center, Tufts University School of Medicine, Boston, MA 02111

Correspondence to Qingshen Gao: q-gao{at}northwestern.edu

In mammalian cells, the centrosome consists of a pair of centrioles and amorphous pericentriolar material. The pair of centrioles, which are the core components of the centrosome, duplicate once per cell cycle. Centrosomes play a pivotal role in orchestrating the formation of the bipolar spindle during mitosis. Recent studies have linked centrosomal activity on centrioles or centriole-associated structures to cytokinesis and cell cycle progression through G1 into the S phase. In this study, we have identified centrobin as a centriole-associated protein that asymmetrically localizes to the daughter centriole. The silencing of centrobin expression by small interfering RNA inhibited centriole duplication and resulted in centrosomes with one or no centriole, demonstrating that centrobin is required for centriole duplication. Furthermore, inhibition of centriole duplication by centrobin depletion led to impaired cytokinesis.

C. Zou, J. Li, and Y. Bai contributed equally to this paper.

Abbreviations used in this paper: cDNA, complementary DNA; HU, hydroxy urea; RNAi, RNA interference; siRNA, small interfering RNA.


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