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Published 21 November 2005. doi:10.1083/jcb.200508127
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 171, Number 4, 593-602
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Article

 The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster

C. Fiona Cullen1, Amy L. Brittle1, Takashi Ito2, and Hiroyuki Ohkura1

1 Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK
2 Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, Nagasaki 852-8523, Japan

Correspondence to Hiroyuki Ohkura: h.ohkura{at}ed.ac.uk

Conventional centrosomes are absent from the spindle in female meiosis in many species, but it is not clear how multiple chromosomes form one shared bipolar spindle without centrosomes. We identified a female sterile mutant in which each bivalent chromosome often forms a separate bipolar metaphase I spindle. Unlike wild type, prophase I chromosomes fail to form a single compact structure within the oocyte nucleus, although the integrity of metaphase I chromosomes appears to be normal. Molecular analysis indicates that the mutant is defective in the conserved kinase nucleosomal histone kinase-1 (NHK-1). Isolation of further alleles and RNA interference in S2 cells demonstrated that NHK-1 is also required for mitotic progression. NHK-1 itself is phosphorylated in mitosis and female meiosis, suggesting that this kinase is part of the regulatory system coordinating progression of mitosis and meiosis.

Abbreviations used in this paper: CNS, central nervous system; D-TACC, Drosophila melanogaster–transforming acidic coiled coil; msps, mini spindles; NHK-1, nucleosomal histone kinase-1; RNAi, RNA interference; SNP, single nucleotide polymorphism.


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