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Published 3 January 2006. doi:10.1083/jcb.200508001
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 172, Number 1, 103-113
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Article

Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis



Shihuan Kuang1, Sophie B. Chargé1, Patrick Seale2, Michael Huh1,3, and Michael A. Rudnicki1,2,3

1 Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada K1H 8L6
2 Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1
3 Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada K1H 8M5

Correspondence to Michael A. Rudnicki: mrudnicki{at}ohri.ca

We assessed viable Pax7–/– mice in 129Sv/J background and observed reduced growth and marked muscle wasting together with a complete absence of functional satellite cells. Acute injury resulted in an extreme deficit in muscle regeneration. However, a small number of regenerated myofibers were detected, suggesting the presence of residual myogenic cells in Pax7-deficient muscle. Rare Pax3+/MyoD+ myoblasts were recovered from Pax7–/– muscle homogenates and cultures of myofiber bundles but not from single myofibers free of interstitial tissues. Finally, we identified Pax3+ cells in the muscle interstitial environment and demonstrated that they coexpressed MyoD during regeneration. Sublaminar satellite cells in hind limb muscle did not express detectable levels of Pax3 protein or messenger RNA. Therefore, we conclude that interstitial Pax3+ cells represent a novel myogenic population that is distinct from the sublaminar satellite cell lineage and that Pax7 is essential for the formation of functional myogenic progenitors from sublaminar satellite cells.

S. Kuang and S.B. Chargé contributed equally to this paper.

P. Seale's present address is Dana-Farber Cancer Institute, Boston, MA 02115.

Abbreviations used in this paper: ß-gal, ß-galactosidase; CTX, cardiotoxin; EDL, extensor digitorum longus; MyHC, myosin heavy chain; P, postnatal day; TA, tibialis anterior.


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