JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 3 January 2006. doi:10.1083/jcb.200506057
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 172, Number 1, 139-149
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2130K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gebhardt, A.
Right arrow Articles by Eilers, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gebhardt, A.
Right arrow Articles by Eilers, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Article

Myc regulates keratinocyte adhesion and differentiation via complex formation with Miz1



Anneli Gebhardt1, Michaela Frye3, Steffi Herold2, Salvador Aznar Benitah3, Kristin Braun3, Birgit Samans2, Fiona M. Watt3, Hans-Peter Elsässer1, and Martin Eilers2

1 Institute for Cell Biology, University of Marburg, 35033 Marburg, Germany
2 Institute for Molecular Biology and Tumor Research, University of Marburg, 35033 Marburg, Germany
3 Cancer Research UK London Research Institute, London WC2A 3PX, England, UK

Correspondence to Martin Eilers: eilers{at}imt.uni-marburg.de

Myc plays a key role in homeostasis of the skin. We show that Miz1, which mediates Myc repression of gene expression, is expressed in the epidermal basal layer. A large percentage of genes regulated by the Myc–Miz1 complex in keratinocytes encode proteins involved in cell adhesion, and some, including the {alpha}6 and ß1 integrins, are directly bound by Myc and Miz1 in vivo. Using a Myc mutant deficient in Miz1 binding (MycV394D), we show that Miz1 is required for the effects of Myc on keratinocyte responsiveness to TGF-ß. Myc, but not MycV394D, decreases keratinocyte adhesion and spreading. In reconstituted epidermis, Myc induces differentiation and loss of cell polarization in a Miz1-dependent manner. In vivo, overexpression of ß1 integrins restores basal layer polarity and prevents Myc-induced premature differentiation. Our data show that regulation of cell adhesion is a major function of the Myc–Miz1 complex and suggest that it may contribute to Myc-induced exit from the epidermal stem cell compartment.

A. Gebhardt and M. Frye contributed equally to this paper.

Abbreviations used in this paper: 4-OHT, 4-hydroxy-tamoxifen; cDNA, complementary DNA; ChIP, chromatin immunoprecipitation; DED, de-epidermized dermis.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents