Published 13 February 2006. doi:10.1083/jcb.200511071
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 172, Number 4, 517-528
Drosophila melanogaster
-TuRC is dispensable for targeting
-tubulin to the centrosome and microtubule nucleation
Christel Vérollet1,
Nathalie Colombié1,
Thomas Daubon1,
Henri-Marc Bourbon2,
Michel Wright1, and
Brigitte Raynaud-Messina1
1 Centre de Recherche en Pharmacologie, Santé, UMR 2587, Centre National de la Recherche Scientifique-Pierre Fabre, Institut de Sciences et Technologies du Médicament de Toulouse, 31432 Toulouse, Cedex 4, France
2 Centre de Biologie du Développement, UMR 5547, Centre National de la Recherche Scientifique-Université Paul Sabatier, IFR109, 31062 Toulouse, Cedex 4, France
Correspondence to Brigitte Raynaud-Messina: brigitte.raynaud-messina{at}istmt.cnrs.fr
In metazoans,
-tubulin acts within two main complexes,
-tubulin small complexes (
-TuSCs) and
-tubulin ring complexes (
-TuRCs). In higher eukaryotes, it is assumed that microtubule nucleation at the centrosome depends on
-TuRCs, but the role of
-TuRC components remains undefined.
For the first time, we analyzed the function of all four
-TuRCspecific subunits in Drosophila melanogaster: Dgrip75, Dgrip128, Dgrip163, and Dgp71WD. Grip-motif proteins, but not Dgp71WD, appear to be required for
-TuRC assembly. Individual depletion of
-TuRC components, in cultured cells and in vivo, induces mitotic delay and abnormal spindles. Surprisingly,
-TuSCs are recruited to the centrosomes. These defects are less severe than those resulting from the inhibition of
-TuSC components and do not appear critical for viability. Simultaneous cosilencing of all
-TuRC proteins leads to stronger phenotypes and partial recruitment of
-TuSC. In conclusion,
-TuRCs are required for assembly of fully functional spindles, but we suggest that
-TuSC could be targeted to the centrosomes, which is where basic microtubule assembly activities are maintained.
Abbreviations used in this paper: Cnn, centrosomin; dsRNA, double-stranded RNA;
-TuRC,
-tubulin ring complex;
-TuSC,
-tubulin small complex; P95, probability of 95%; RNAi, RNA interference.

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